Document Detail


Biosynthesis of acetylcholine in turtle photoreceptors.
MedLine Citation:
PMID:  4505678     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For determination of possible neurotransmitters synthesized by photoreceptor cells, turtle retinas were dissociated into single cells with proteolytic enzymes. These cells were partially separated by velocity sedimentation to yield a fraction rich in photoreceptors. Individual photoreceptor cells were then sucked into a micropipette and incubated with labeled precursors of known or suspected neurotransmitters. After incubation, the radioactive products were analyzed by high-voltage electrophoresis. Of all the chemicals tested, turtle photoreceptor cells synthesized only acetylcholine, suggesting that these cells may be cholinergic.
Authors:
D M Lam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Jul 
Date Detail:
Created Date:  1972-09-22     Completed Date:  1972-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1987-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / biosynthesis*
Acetylcholinesterase
Acyltransferases / analysis
Aminobutyric Acids / biosynthesis
Animals
Carbon Isotopes
Choline / metabolism
Coenzyme A
Dopamine / biosynthesis
Electrophoresis, Paper
Glutamates / metabolism
Papain
Photoreceptor Cells / enzymology,  metabolism*
Retina / cytology
Tritium
Tryptophan / metabolism
Turtles*
Tyrosine / metabolism
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Aminobutyric Acids; 0/Carbon Isotopes; 0/Glutamates; 10028-17-8/Tritium; 51-84-3/Acetylcholine; 55520-40-6/Tyrosine; 62-49-7/Choline; 73-22-3/Tryptophan; 85-61-0/Coenzyme A; EC 2.3.-/Acyltransferases; EC 3.1.1.7/Acetylcholinesterase; EC 3.4.22.2/Papain
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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