Document Detail


Biosynthesis of rhizocticins, antifungal phosphonate oligopeptides produced by Bacillus subtilis ATCC6633.
MedLine Citation:
PMID:  20142038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhizocticins are phosphonate oligopeptide antibiotics containing the C-terminal nonproteinogenic amino acid (Z)-l-2-amino-5-phosphono-3-pentenoic acid (APPA). Here we report the identification and characterization of the rhizocticin biosynthetic gene cluster (rhi) in Bacillus subtilis ATCC6633. Rhizocticin B was heterologously produced in the nonproducer strain Bacillus subtilis 168. A biosynthetic pathway is proposed on the basis of bioinformatics analysis of the rhi genes. One of the steps during the biosynthesis of APPA is an unusual aldol reaction between phosphonoacetaldehyde and oxaloacetate catalyzed by an aldolase homolog RhiG. Recombinant RhiG was prepared, and the product of an in vitro enzymatic conversion was characterized. Access to this intermediate allows for biochemical characterization of subsequent steps in the pathway.
Authors:
Svetlana A Borisova; Benjamin T Circello; Jun Kai Zhang; Wilfred A van der Donk; William W Metcalf
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Chemistry & biology     Volume:  17     ISSN:  1879-1301     ISO Abbreviation:  Chem. Biol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-09     Completed Date:  2010-05-04     Revised Date:  2014-01-16    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-37     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FJ935779
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antifungal Agents / metabolism*
Bacillus subtilis / genetics,  metabolism*
Bacterial Proteins / metabolism
Computational Biology
Molecular Sequence Data
Multigene Family*
Oligopeptides / genetics*,  metabolism*
Organophosphonates / metabolism*
Organophosphorus Compounds / metabolism
Phosphotransferases (Phosphomutases) / metabolism
Grant Support
ID/Acronym/Agency:
P01 GM 077596/GM/NIGMS NIH HHS; P01 GM077596/GM/NIGMS NIH HHS; P01 GM077596-03/GM/NIGMS NIH HHS; T32 GM070421/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Bacterial Proteins; 0/Oligopeptides; 0/Organophosphonates; 0/Organophosphorus Compounds; 0/rhizocticin B; EC 5.4.2.-/Phosphotransferases (Phosphomutases); EC 5.4.2.9/phosphoenolpyruvate mutase
Comments/Corrections

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