| Biosynthesis of rhizocticins, antifungal phosphonate oligopeptides produced by Bacillus subtilis ATCC6633. | |
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MedLine Citation:
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PMID: 20142038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rhizocticins are phosphonate oligopeptide antibiotics containing the C-terminal nonproteinogenic amino acid (Z)-l-2-amino-5-phosphono-3-pentenoic acid (APPA). Here we report the identification and characterization of the rhizocticin biosynthetic gene cluster (rhi) in Bacillus subtilis ATCC6633. Rhizocticin B was heterologously produced in the nonproducer strain Bacillus subtilis 168. A biosynthetic pathway is proposed on the basis of bioinformatics analysis of the rhi genes. One of the steps during the biosynthesis of APPA is an unusual aldol reaction between phosphonoacetaldehyde and oxaloacetate catalyzed by an aldolase homolog RhiG. Recombinant RhiG was prepared, and the product of an in vitro enzymatic conversion was characterized. Access to this intermediate allows for biochemical characterization of subsequent steps in the pathway. |
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Authors:
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Svetlana A Borisova; Benjamin T Circello; Jun Kai Zhang; Wilfred A van der Donk; William W Metcalf |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Chemistry & biology Volume: 17 ISSN: 1879-1301 ISO Abbreviation: Chem. Biol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-09 Completed Date: 2010-05-04 Revised Date: 2011-07-20 |
Medline Journal Info:
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Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 28-37 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FJ935779 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antifungal Agents / metabolism* Bacillus subtilis / genetics, metabolism* Bacterial Proteins / metabolism Computational Biology Molecular Sequence Data Multigene Family* Oligopeptides / genetics*, metabolism* Organophosphorus Compounds / metabolism Phosphonic Acids / metabolism* Phosphotransferases (Phosphomutases) / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 GM 077596/GM/NIGMS NIH HHS; P01 GM077596-03/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/Bacterial Proteins; 0/Oligopeptides; 0/Organophosphorus Compounds; 0/Phosphonic Acids; 0/rhizocticin B; EC 5.4.2.-/Phosphotransferases (Phosphomutases); EC 5.4.2.9/phosphoenolpyruvate mutase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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