| Biosynthesis of monomers for plastics from renewable oils. | |
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MedLine Citation:
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PMID: 20936848 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Omega-hydroxyfatty acids are excellent monomers for synthesizing a unique family of polyethylene-like biobased plastics. However, ω-hydroxyfatty acids are difficult and expensive to prepare by traditional organic synthesis, precluding their use in commodity materials. Here we report the engineering of a strain of the diploid yeast Candida tropicalis to produce commercially viable yields of ω-hydroxyfatty acids. To develop the strain we identified and eliminated 16 genes encoding 6 cytochrome P450s, 4 fatty alcohol oxidases, and 6 alcohol dehydrogenases from the C. tropicalis genome. We also show that fatty acids with different chain lengths and degrees of unsaturation can be more efficiently oxidized by expressing different P450s within this strain background. Biocatalysis using engineered C. tropicalis is thus a potentially attractive biocatalytic platform for producing commodity chemicals from renewable resources. |
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Authors:
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Wenhua Lu; Jon E Ness; Wenchun Xie; Xiaoyan Zhang; Jeremy Minshull; Richard A Gross |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 132 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 15451-5 Citation Subset: IM |
Affiliation:
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Polytechnic Institute of New York University, 6 Metrotech Center, Brooklyn, New York 11201, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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