Document Detail

Biosynthesis of Iminodiacetic Acid from Iminodiacetonitrile by Immobilized Recombinant Escherichia coli Harboring Nitrilase.
MedLine Citation:
PMID:  22441427     Owner:  NLM     Status:  Publisher    
Iminodiacetic acid (IDA) is widely used as an intermediate in the manufacture of chelating agents, glyphosate herbicides and surfactants. In the current work, the fragment with the length of 1,110 bp encoding the Acidovorax facilis nitrilase was obtained. The recombinant nitrilase expressed in Escherichia coli BL21 (DE3) was successfully used in the production of IDA from iminodiacetonitrile. To improve the stability of operation, the recombinant cells were entrapped in polyvinyl alcohol (PVA) and sodium alginate (SA) copolymer. The maximum relative nitrilase activity with 98.1% was further observed at 1.0% SA, 8.0% PVA, 1.0% CaCl(2), and 5.0% wet cells, under conditions of 1.0% iminodiacetonitrile in distilled water and a temperature of 40°C, respectively. The entrapped cells facilitated easy separation and good recycling compared with free cells. Moreover, the immobilized cells showed good operation and storage stability. This report is the first to describe IDA preparation using immobilized recombinant E. coli harboring nitrilase.
Zhi-Qiang Liu; Ming Zhou; Xin-Hong Zhang; Jian-Miao Xu; Ya-Ping Xue; Yu-Guo Zheng
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-22
Journal Detail:
Title:  Journal of molecular microbiology and biotechnology     Volume:  22     ISSN:  1660-2412     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892561     Medline TA:  J Mol Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  35-47     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, China.
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