Document Detail

Biosynthesis of D-aspartate in mammalian cells.
MedLine Citation:
PMID:  9742929     Owner:  NLM     Status:  MEDLINE    
In this communication, we demonstrate that D-aspartate (D-Asp) is synthesized in pheochromocytoma cells (PC12). To our knowledge this is the first report of biosynthesis of D-Asp in mammalian cells. Synthesis of D-Asp was demonstrated by its time-dependent accumulation in the cell culture, and by the fact that this accumulation was proportional to the number of inoculated cells. D-Asp in PC12 cells was identified by (i) co-elution with authentic D-Asp on two different HPLC columns, an octadesyl silica column and a Pirkle-type chiral column, (ii) reversed elution order of D-Asp and L-Asp on another Pirkle-type chiral column with an opposite configuration, and (iii) sensitivity to D-Asp oxidase. In the cells the amount of D-Asp was approx. 12-14% of total Asp and no other investigated D-amino acid was detected. The amount of D-Asp did not increase during the culture of mouse 3T3 fibroblasts and human neuroblastoma NB-1 cells. Immunocytochemical staining with anti-D-Asp antiserum demonstrated that D-Asp synthesized is present in the cytoplasm of the cells.
Z Long; H Homma; J A Lee; T Fukushima; T Santa; T Iwatsubo; R Yamada; K Imai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  434     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-10-08     Completed Date:  1998-10-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  231-5     Citation Subset:  IM    
Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
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MeSH Terms
3T3 Cells
Aspartic Acid / biosynthesis*,  chemistry,  isolation & purification
Chromatography, High Pressure Liquid
Cytoplasm / chemistry
PC12 Cells
Tumor Cells, Cultured
Reg. No./Substance:
56-84-8/Aspartic Acid

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