Document Detail


Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent.
MedLine Citation:
PMID:  23028854     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C(8:0)) to oleic acid (C(18:1)) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the (1)H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T(d)) of 264.6 to 318.8 (± 0.2) (o)C, melting temperature (T(m)) of 43. (± 0.2) (o)C, glass transition temperature (T(g)) of -1.0 (± 0.2) (o)C and apparent melting enthalpy of fusion (ΔH(f)) of 100.9 (± 0.1) J g(-1).
Authors:
Ahmad Mohammed Gumel; Mohamad Suffian Mohamad Annuar; Thorsten Heidelberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-20
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-02-28     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e45214     Citation Subset:  IM    
Affiliation:
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
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MeSH Terms
Descriptor/Qualifier:
Arecaceae / microbiology
Carbon / metabolism
Fatty Acids / analysis*
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Molecular Conformation
Molecular Weight
Phase Transition
Plant Oils / chemistry*
Polyhydroxyalkanoates / biosynthesis*,  isolation & purification*
Pseudomonas putida / metabolism*
Temperature
Thermodynamics
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Plant Oils; 0/Polyhydroxyalkanoates; 7440-44-0/Carbon; 8002-75-3/palm oil
Comments/Corrections

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