Document Detail


Biosynthesis of [1-15N] L-tryptophan from 15N labeled anthranilic acid by fermentation of Candida utilis mutant.
MedLine Citation:
PMID:  18235989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method for synthesizing the labeled L-tryptophan is described in this work. L-tryptophan, labeled with 98% (15)N at position 1 was synthesized from the labeled anthranilic acid using Candida utilis mutants. The conversion ratio of (15)N of 50% was achieved. The labeled anthranilic acid was synthesized by [(15)N] phthalimide that was prepared by 99.34% [(15)N] urea and phthalic anhydride in ortho-xylene medium at 140 degrees C and under atmospheric pressure.
Authors:
Zhanfeng Liu; Qipeng Yuan; Wenchuan Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-31
Journal Detail:
Title:  Amino acids     Volume:  36     ISSN:  1438-2199     ISO Abbreviation:  Amino Acids     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-06     Completed Date:  2009-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  71-3     Citation Subset:  IM    
Affiliation:
College of Life Science and Technology, Beijing University of Chemical Technology, P. O. Box 75, 100029, Beijing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Anthranilic Acids / chemistry,  metabolism*
Candida / genetics,  metabolism*
Molecular Structure
Mutation / genetics
Nitrogen Isotopes
Tryptophan / biosynthesis*,  chemistry
Chemical
Reg. No./Substance:
0/Anthranilic Acids; 0/Nitrogen Isotopes; 118-92-3/anthranilic acid; 73-22-3/Tryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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