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Biosurfactant production in sugar beet molasses by some Pseudomonas spp.
MedLine Citation:
PMID:  20112880     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study rhamnolipid biosurfactant production was investigated in eighteen strains of Pseudomonas spp.. Rhamnolipid by these strains was determined by a spectrophotometric method in nutrient broth medium (NB). From the 18 strains screened, two Pseudomonas strains (Pseudomonas luteola B17 and Pseudomonas putida B12) which had produced the highest percentage yield of rhamnolipid were examined for rhamnolipid production at different incubation times (24, 48 and 72 hr) and different sugar beet molasses concentrations [1-5% w/v concentration (1-5 g molasses/100 ml water)]. The rhamnolipid production increased with the increase in the concentration of molasses and maximum production occurred when 5 % (w/v) of molasses were used. At the same time, maximum rhamnolipid production occurred after 72 hr of incubation. When the amount of rhamnolipid produced at different incubation times (24, 48 and 72 hr) and with different concentrations of molasses [1-5 % w/v concentration (1-5 g molasses/100 ml water)] by Pseudomonas spp.; was compared, no significant difference in amount of production was seen. These studies show that the waste product from sugar industry may be suggested for important biotechnological processes such as rhamnolipid production.
Authors:
Dilsad Onbasli; Belma Aslim
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of environmental biology / Academy of Environmental Biology, India     Volume:  30     ISSN:  0254-8704     ISO Abbreviation:  J Environ Biol     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8300544     Medline TA:  J Environ Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  161-3     Citation Subset:  IM    
Affiliation:
Deparment of Biology, Faculty of Science and Arts, Kastamonu University, Kastamonu, Turkey. donbasili@kastamonu.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Beta vulgaris / chemistry*
Carbon / metabolism
Glycolipids / biosynthesis*
Molasses*
Pseudomonas / growth & development,  metabolism*
Surface-Active Agents / metabolism*
Chemical
Reg. No./Substance:
0/Glycolipids; 0/Surface-Active Agents; 0/rhamnolipid; 7440-44-0/Carbon

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