Document Detail


Biostimulation induces syntrophic interactions that impact C, S and N cycling in a sediment microbial community.
MedLine Citation:
PMID:  23190730     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stimulation of subsurface microorganisms to induce reductive immobilization of metals is a promising approach for bioremediation, yet the overall microbial community response is typically poorly understood. Here we used proteogenomics to test the hypothesis that excess input of acetate activates complex community functioning and syntrophic interactions among autotrophs and heterotrophs. A flow-through sediment column was incubated in a groundwater well of an acetate-amended aquifer and recovered during microbial sulfate reduction. De novo reconstruction of community sequences yielded near-complete genomes of Desulfobacter (Deltaproteobacteria), Sulfurovum- and Sulfurimonas-like Epsilonproteobacteria and Bacteroidetes. Partial genomes were obtained for Clostridiales (Firmicutes) and Desulfuromonadales-like Deltaproteobacteria. The majority of proteins identified by mass spectrometry corresponded to Desulfobacter-like species, and demonstrate the role of this organism in sulfate reduction (Dsr and APS), nitrogen fixation and acetate oxidation to CO2 during amendment. Results indicate less abundant Desulfuromonadales, and possibly Bacteroidetes, also actively contributed to CO2 production via the tricarboxylic acid (TCA) cycle. Proteomic data indicate that sulfide was partially re-oxidized by Epsilonproteobacteria through nitrate-dependent sulfide oxidation (using Nap, Nir, Nos, SQR and Sox), with CO2 fixed using the reverse TCA cycle. We infer that high acetate concentrations, aimed at stimulating anaerobic heterotrophy, led to the co-enrichment of, and carbon fixation in Epsilonproteobacteria. Results give an insight into ecosystem behavior following addition of simple organic carbon to the subsurface, and demonstrate a range of biological processes and community interactions were stimulated.
Authors:
Kim M Handley; Nathan C VerBerkmoes; Carl I Steefel; Kenneth H Williams; Itai Sharon; Christopher S Miller; Kyle R Frischkorn; Karuna Chourey; Brian C Thomas; Manesh B Shah; Philip E Long; Robert L Hettich; Jillian F Banfield
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-29
Journal Detail:
Title:  The ISME journal     Volume:  7     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-09-30     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  800-16     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteroidetes / classification,  isolation & purification,  metabolism
Biodegradation, Environmental
Carbon
Deltaproteobacteria / classification,  isolation & purification,  metabolism*
Ecosystem
Epsilonproteobacteria / classification,  isolation & purification,  metabolism*
Fresh Water / microbiology*
Geologic Sediments / microbiology*
Groundwater / microbiology*
Nitrogen Cycle
Oxidation-Reduction
Proteomics*
Sulfur
Chemical
Reg. No./Substance:
70FD1KFU70/Sulfur; 7440-44-0/Carbon
Comments/Corrections

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