Document Detail


Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity.
MedLine Citation:
PMID:  21047668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biosorption of uranium(VI) by the green alga Chlorella vulgaris was studied at varying uranium concentrations from 5 μM to 1mM, and in the environmentally relevant pH range of 4.4 to 7.0. Living cells bind in a 0.1mM uranium solution at pH 4.4 within 5 min 14.3 ± 5.5 mg U/g dry biomass and dead cells 28.3 ± 0.6 mg U/g dry biomass which corresponds to 45% and 90% of total uranium in solution, respectively. During 96 h of incubation with uranium initially living cells died off and with 26.6 ± 2.1 mg U/g dry biomass bound similar amounts of uranium compared to dead cells, binding 27.0 ± 0.7 mg U/g dry biomass. In both cases, these amounts correspond to around 85% of the initially applied uranium. Interestingly, at a lower and more environmentally relevant uranium concentration of 5 μM, living cells firstly bind with 1.3 ± 0.2 mg U/g dry biomass to 1.4 ± 0.1 mg U/g dry biomass almost all uranium within the first 5 min of incubation. But then algal cells again mobilize up to 80% of the bound uranium during ongoing incubation in the time from 48 h to 96 h. The release of metabolism related substances is suggested to cause this mobilization of uranium. As potential leachates for algal-bound uranium oxalate, citrate and ATP were tested and found to be able to mobilize more than 50% of the algal-bound uranium within 24h. Differences in complexation of uranium by active and inactive algae cells were investigated with a combination of time-resolved laser-induced fluorescence spectroscopy (TRLFS), extended X-ray absorption fine structure (EXAFS) spectroscopy and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Obtained results demonstrated an involvement of carboxylic and organic/inorganic phosphate groups in the uranium complexation with varying contributions dependent on cell status, uranium concentration and pH.
Authors:
M Vogel; A Günther; A Rossberg; B Li; G Bernhard; J Raff
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Publication Detail:
Type:  Journal Article     Date:  2010-11-02
Journal Detail:
Title:  The Science of the total environment     Volume:  409     ISSN:  1879-1026     ISO Abbreviation:  Sci. Total Environ.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-06     Completed Date:  2011-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0330500     Medline TA:  Sci Total Environ     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  384-95     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Forschungszentrum Dresden-Rossendorf e. V., Institute of Radiochemistry, P.O. Box 510119, 01314 Dresden, Germany. M.Vogel@fzd.de
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MeSH Terms
Descriptor/Qualifier:
Chlorella vulgaris / chemistry,  metabolism*,  radiation effects
Fresh Water / chemistry
Hydrogen-Ion Concentration
Uranium / chemistry,  metabolism*,  toxicity
Water Pollutants, Radioactive / chemistry,  metabolism*,  toxicity
Chemical
Reg. No./Substance:
0/Water Pollutants, Radioactive; 7440-61-1/Uranium

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