| Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity. | |
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MedLine Citation:
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PMID: 21047668 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biosorption of uranium(VI) by the green alga Chlorella vulgaris was studied at varying uranium concentrations from 5 μM to 1mM, and in the environmentally relevant pH range of 4.4 to 7.0. Living cells bind in a 0.1mM uranium solution at pH 4.4 within 5 min 14.3 ± 5.5 mg U/g dry biomass and dead cells 28.3 ± 0.6 mg U/g dry biomass which corresponds to 45% and 90% of total uranium in solution, respectively. During 96 h of incubation with uranium initially living cells died off and with 26.6 ± 2.1 mg U/g dry biomass bound similar amounts of uranium compared to dead cells, binding 27.0 ± 0.7 mg U/g dry biomass. In both cases, these amounts correspond to around 85% of the initially applied uranium. Interestingly, at a lower and more environmentally relevant uranium concentration of 5 μM, living cells firstly bind with 1.3 ± 0.2 mg U/g dry biomass to 1.4 ± 0.1 mg U/g dry biomass almost all uranium within the first 5 min of incubation. But then algal cells again mobilize up to 80% of the bound uranium during ongoing incubation in the time from 48 h to 96 h. The release of metabolism related substances is suggested to cause this mobilization of uranium. As potential leachates for algal-bound uranium oxalate, citrate and ATP were tested and found to be able to mobilize more than 50% of the algal-bound uranium within 24h. Differences in complexation of uranium by active and inactive algae cells were investigated with a combination of time-resolved laser-induced fluorescence spectroscopy (TRLFS), extended X-ray absorption fine structure (EXAFS) spectroscopy and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Obtained results demonstrated an involvement of carboxylic and organic/inorganic phosphate groups in the uranium complexation with varying contributions dependent on cell status, uranium concentration and pH. |
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Authors:
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M Vogel; A Günther; A Rossberg; B Li; G Bernhard; J Raff |
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Publication Detail:
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Type: Journal Article Date: 2010-11-02 |
Journal Detail:
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Title: The Science of the total environment Volume: 409 ISSN: 1879-1026 ISO Abbreviation: Sci. Total Environ. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-06 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0330500 Medline TA: Sci Total Environ Country: Netherlands |
Other Details:
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Languages: eng Pagination: 384-95 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Forschungszentrum Dresden-Rossendorf e. V., Institute of Radiochemistry, P.O. Box 510119, 01314 Dresden, Germany. M.Vogel@fzd.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chlorella vulgaris
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chemistry,
metabolism*,
radiation effects Fresh Water / chemistry Hydrogen-Ion Concentration Uranium / chemistry, metabolism*, toxicity Water Pollutants, Radioactive / chemistry, metabolism*, toxicity |
| Chemical | |
Reg. No./Substance:
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0/Water Pollutants, Radioactive; 7440-61-1/Uranium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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