Document Detail

Biosensors and flow-through system for the determination of creatinine in hemodialysate.
MedLine Citation:
PMID:  11936100     Owner:  NLM     Status:  MEDLINE    
Biosensors for the determination of creatinine have been developed and integrated into a flow-through system. The sensors are based on a screen-printed three electrode transducer with a platinum working electrode. Applying the multi-enzyme sequence of creatininase (CA), creatinase (CI) and sarcosine oxidase (SO) hydrogen peroxide has been detected amperometrically. An optimal enzyme load was found to be 4.4 U/0.28 U/0.20 U (CA/CI/SO) and 0.28 U/0.20 U (CI/SO) per electrode for the creatinine sensor and for the creatine sensor, respectively. Among a variety of polymers Nafion has shown the highest efficiency to exclude interfering substances like ascorbic acid, acetaminophen and uric acid. First determinations of creatinine in dialysate samples obtained during hemodialysis treatments have shown a good correlation to the conventional methods, the Jaffé reaction (y=0.945x+ 2.8, R=0.9882, n=9) and the enzymatic photometric method (y=0.891x+3.5, R=0.9917, n=9).
Ansgar Erlenkötter; Manfred Fobker; Gabriele-Christine Chemnitius
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-12-15
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  372     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-04-08     Completed Date:  2003-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  284-92     Citation Subset:  IM    
Institute for Chemical and Biochemical Sensor Research (ICB), Münster, Germany.
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MeSH Terms
Amidohydrolases / metabolism
Biosensing Techniques / instrumentation,  methods*,  standards
Creatinine / analysis*
Disposable Equipment
Drug Stability
Enzymes, Immobilized
Hemodialysis Solutions / analysis*
Oxidoreductases, N-Demethylating / metabolism
Reproducibility of Results
Sarcosine Oxidase
Sensitivity and Specificity
Ureohydrolases / metabolism
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Hemodialysis Solutions; 60-27-5/Creatinine; EC 1.5.-/Oxidoreductases, N-Demethylating; EC Oxidase; EC 3.5.-/Amidohydrolases; EC; EC 3.5.3.-/Ureohydrolases; EC

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