| Bioproperties and purification of xylanase from Bacillus sp. YJ6. | |
| | |
MedLine Citation:
|
PMID: 19911836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To characterize the xylanase from Bacillus sp. YJ6, broth after 4 days incubation at 25 degrees C was collected and purified to electrophoretical homogeneity after Sephacryl S-100 HR chromatograph. About 3.5% recovery and 678.1 purification fold were achieved. The purified xylanase, with a Mw of 19 kDa, had an optimal pH and temperature at 5.0 and 50 degrees C, respectively, and was stable at pH 5.0-9.0 or <50 degrees C. It was inhibited by Cu2+, Fe3+, Hg2+, phenylmethyl sulfonyl fluoride (PMSF), N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), N-ethylmaleimide (NEM), and leupeptin but activated by K+, Na+, Co2+, Mg2+, beta-mercaptoethanol (beta-ME), and glutathione (GSH). The purified xylanase had high specificity to beechwood, birchwood, and oat spelt xylans. The DNA fragment encoding this xylanase, corresponding to 213 amino acids, exhibited about 95% homology with seven strains of Bacillus in the NCBI database. |
| | |
Authors:
|
Li-Jung Yin; Hsin-Hung Lin; Yen-I Chiang; Shann-Tzong Jiang |
Related Documents
:
|
21659966 - Lichtheimia blakesleeana as a new potencial producer of phytase and xylanase. 18366686 - Production of functionally active penicillium chrysogenum isopenicillin n synthase in t... 6942416 - Angiogenic activity from bovine retina: partial purification and characterization. 15969016 - Purification and properties of bacillus subtilis sa-22 endo-1, 4-beta-d-mannanase. 15581796 - A novel serine protease complexed with alpha2-macroglobulin from skeletal muscle of liz... 6246656 - Cyanides and their toxicity: a literature review. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-06 Completed Date: 2010-03-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 557-62 Citation Subset: IM |
Affiliation:
|
Department of Sea Food Science, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung 81143, Taiwan. ljyin@mail.nkmu.edu.tw |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Bacillus / chemistry, enzymology*, genetics Bacterial Proteins / chemistry*, genetics, isolation & purification*, metabolism Base Sequence Endo-1,4-beta Xylanases / chemistry*, genetics, isolation & purification*, metabolism Enzyme Stability Molecular Sequence Data Molecular Weight Substrate Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; EC 3.2.1.8/Endo-1,4-beta Xylanases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Controllable Nanofabrication of Aggregate-like Nanoparticle Substrates and Evaluation for Surface-En...
Next Document: Novel Nucleophilic Heterocyclic Carbene Mediated Stereoselective Conjugate Addition of Enals to Nitr...