Document Detail

Bioprinting vessel-like constructs using hyaluronan hydrogels crosslinked with tetrahedral polyethylene glycol tetracrylates.
MedLine Citation:
PMID:  20546891     Owner:  NLM     Status:  MEDLINE    
Bioprinting enables deposition of cells and biomaterials into spatial orientations and complexities that mirror physiologically relevant geometries. To facilitate the development of bioartificial vessel-like grafts, two four-armed polyethylene glycol (PEG) derivatives with different PEG chain lengths, TetraPEG8 and TetraPEG13, were synthesized from tetrahedral pentaerythritol derivatives. The TetraPEGs are unique multi-armed PEGs with a compact and symmetrical core. The TetraPEGs were converted to tetra-acrylate derivatives (TetraPAcs) which were used in turn to co-crosslink thiolated hyaluronic acid and gelatin derivatives into extrudable hydrogels for printing tissue constructs. First, the hydrogels produced by TetraPAc crosslinking showed significantly higher shear storage moduli when compared to PEG diacrylate (PEGDA)-crosslinked synthetic extracellular matrices (sECMs) of similar composition. These stiffer hydrogels have rheological properties more suited to bioprinting high-density cell suspensions. Second, TetraPAc-crosslinked sECMs were equivalent or superior to PEGDA-crosslinked gels in supporting cell growth and proliferation. Third, the TetraPac sECMs were employed in a proof-of-concept experiment by encapsulation of NIH 3T3 cells in sausage-like hydrogel macrofilaments. These macrofilaments were then printed into tubular tissue constructs by layer-by-layer deposition using the Fab@Home printing system. LIVE/DEAD viability/cytotoxicity-stained cross-sectional images showed the bioprinted cell structures to be viable in culture for up to 4 weeks with little evidence of cell death. Thus, biofabrication of cell suspensions in TetraPAc sECMs demonstrates the feasibility of building bioartificial blood vessel-like constructs for research and potentially clinical uses.
Aleksander Skardal; Jianxing Zhang; Glenn D Prestwich
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-09-20     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  6173-81     Citation Subset:  IM    
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MeSH Terms
Biocompatible Materials / chemical synthesis
Blood Vessels / drug effects,  physiology*
Cell Proliferation / drug effects
Cross-Linking Reagents / pharmacology*
Hep G2 Cells
Hyaluronic Acid / pharmacology*
Hydrogels / pharmacology*
Materials Testing
NIH 3T3 Cells
Polyethylene Glycols / chemical synthesis,  chemistry,  pharmacology*
Rheology / drug effects
Stress, Mechanical
Tissue Engineering / methods*
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Cross-Linking Reagents; 0/Hydrogels; 0/Polyethylene Glycols; 9004-61-9/Hyaluronic Acid

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