Document Detail

Biopolymer system for cell recovery from microfluidic cell capture devices.
MedLine Citation:
PMID:  22414137     Owner:  NLM     Status:  MEDLINE    
Microfluidic systems for affinity-based cell isolation have emerged as a promising approach for the isolation of specific cells from complex matrices (i.e., circulating tumor cells in whole blood). However, these technologies remain limited by the lack of reliable methods for the innocuous recovery of surface captured cells. Here, we present a biofunctional sacrificial hydrogel coating for microfluidic chips that enables the highly efficient release of isolated cells (99% ± 1%) following gel dissolution. This covalently cross-linked alginate biopolymer system is stable in a wide variety of physiologic solutions (including EDTA treated whole blood) and may be rapidly degraded via backbone cleavage with alginate lyase. The capture and release of EpCAM expressing cancer cells using this approach was found to have no significant effect on cell viability or proliferative potential, and recovered cells were demonstrated to be compatible with downstream immunostaining and FISH analysis.
Ajay M Shah; Min Yu; Zev Nakamura; Jordan Ciciliano; Matthew Ulman; Kenneth Kotz; Shannon L Stott; Shyamala Maheswaran; Daniel A Haber; Mehmet Toner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-04-03
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-17     Completed Date:  2012-09-18     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3682-8     Citation Subset:  IM    
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MeSH Terms
Alginates / chemistry*
Biopolymers / chemistry*
Carbohydrate Sequence
Cell Line, Tumor
Cell Survival
Cell Tracking*
Hydrogel / chemistry*
Microfluidic Analytical Techniques
Molecular Sequence Data
Molecular Structure
Surface Properties
Grant Support
P41 EB002503/EB/NIBIB NIH HHS; R01 EB008047/EB/NIBIB NIH HHS; U01 EB012493/EB/NIBIB NIH HHS; U01 EB012493-03/EB/NIBIB NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Alginates; 0/Biopolymers; 25852-47-5/Hydrogel

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