| Biophysical properties of mitochondrial fusion events in pancreatic beta-cells and cardiac cells unravel potential control mechanisms of its selectivity. | |
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MedLine Citation:
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PMID: 20445168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies in various types of cells find that, on average, each mitochondrion becomes involved in a fusion event every 15 min, depending on the cell type. As most contact events do not result in mitochondrial fusion, it is expected that properties of the individual mitochondrion determine the likelihood of a fusion event. However, apart from membrane potential, the properties that influence the likelihood of entering a fusion event are not known. Here, we tag and track individual mitochondria in H9c2, INS1, and primary beta-cells and determine the biophysical properties that increase the likelihood of a fusion event. We found that the probability for fusion is independent of contact duration and organelle dimensions, but it is influenced by organelle motility. Furthermore, the history of a previous fusion event of the individual mitochondrion influenced both the likelihood for a subsequent fusion event, as well as the site on the mitochondrion at which the fusion occurred. These observations unravel the specific properties that distinguish mitochondria that will enter fusion events from the ones that will not. Altogether, these properties may help to elucidate the molecular mechanisms that regulate fusion at the level of the single mitochondrion. |
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Authors:
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Gilad Twig; Xingguo Liu; Marc Liesa; Jakob D Wikstrom; Anthony J A Molina; Guy Las; Gal Yaniv; György Hajnóczky; Orian S Shirihai |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-05-05 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 299 ISSN: 1522-1563 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-08-19 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C477-87 Citation Subset: IM |
Affiliation:
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Evans Biomedical Research Center, Boston University, Boston, Massachusetts 02118, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cells, Cultured Insulin-Secreting Cells / cytology, physiology*, ultrastructure Male Membrane Fusion / physiology Membrane Potential, Mitochondrial / physiology* Mice Mice, Inbred C57BL Mitochondria / physiology*, ultrastructure Myocytes, Cardiac / cytology, physiology*, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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AA017773/AA/NIAAA NIH HHS; R01 AA017773/AA/NIAAA NIH HHS; R01 DK035914-26/DK/NIDDK NIH HHS; R01 DK056690-12/DK/NIDDK NIH HHS; R01DK-074778/DK/NIDDK NIH HHS; R01HL-071629-03/HL/NHLBI NIH HHS |
| Comments/Corrections | |
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