Document Detail


Biophysical properties of mitochondrial fusion events in pancreatic beta-cells and cardiac cells unravel potential control mechanisms of its selectivity.
MedLine Citation:
PMID:  20445168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies in various types of cells find that, on average, each mitochondrion becomes involved in a fusion event every 15 min, depending on the cell type. As most contact events do not result in mitochondrial fusion, it is expected that properties of the individual mitochondrion determine the likelihood of a fusion event. However, apart from membrane potential, the properties that influence the likelihood of entering a fusion event are not known. Here, we tag and track individual mitochondria in H9c2, INS1, and primary beta-cells and determine the biophysical properties that increase the likelihood of a fusion event. We found that the probability for fusion is independent of contact duration and organelle dimensions, but it is influenced by organelle motility. Furthermore, the history of a previous fusion event of the individual mitochondrion influenced both the likelihood for a subsequent fusion event, as well as the site on the mitochondrion at which the fusion occurred. These observations unravel the specific properties that distinguish mitochondria that will enter fusion events from the ones that will not. Altogether, these properties may help to elucidate the molecular mechanisms that regulate fusion at the level of the single mitochondrion.
Authors:
Gilad Twig; Xingguo Liu; Marc Liesa; Jakob D Wikstrom; Anthony J A Molina; Guy Las; Gal Yaniv; György Hajnóczky; Orian S Shirihai
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-05-05
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  299     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2010-08-19     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C477-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cells, Cultured
Insulin-Secreting Cells / cytology,  physiology*,  ultrastructure
Male
Membrane Fusion / physiology
Membrane Potential, Mitochondrial / physiology*
Mice
Mice, Inbred C57BL
Mitochondria / physiology*,  ultrastructure
Myocytes, Cardiac / cytology,  physiology*,  ultrastructure
Grant Support
ID/Acronym/Agency:
AA017773/AA/NIAAA NIH HHS; R01 AA017773/AA/NIAAA NIH HHS; R01 DK035914/DK/NIDDK NIH HHS; R01 DK056690/DK/NIDDK NIH HHS; R01DK-074778/DK/NIDDK NIH HHS; R01HL-071629-03/HL/NHLBI NIH HHS
Comments/Corrections

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