Document Detail


Biophysical optima for metabolism of Mycobacterium leprae.
MedLine Citation:
PMID:  3290244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The metabolic response of freshly harvested, nude-mouse-derived Mycobacterium leprae to biophysical parameters was studied to facilitate an understanding of axenic culture requirements. Quantitation of intracellular ATP and the rate of [U-14C]palmitic acid incorporation into phenolic glycolipid I (PGL-I) were used as metabolic indicators after axenic incubation in modified Dubos medium under various biophysical conditions. PGL-I synthesis was optimal at 33 degrees C, whereas ATP was optimally maintained at less than or equal to 33 degrees C. Both metabolic indices showed sharp reductions at 37 degrees C. After 5 days of incubation, PGL-I synthesis and ATP maintenance showed pH optima of 5.1 to 5.6, with the higher value appearing optimal for ATP maintenance after extended incubation. Metabolic activity was negatively affected by strong reducing agents, and ATP maintenance was optimal when the gaseous environment was maintained at 2.5 to 10% oxygen. The results may partially explain the failure to cultivate the leprosy bacillus in vitro.
Authors:
S G Franzblau; E B Harris
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  26     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-08-02     Completed Date:  1988-08-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1124-9     Citation Subset:  IM    
Affiliation:
Laboratory Research Branch, Gillis W. Long Hansen's Disease Center, Carville, Louisiana 70721.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Glycolipids / biosynthesis
Hydrogen-Ion Concentration
Mycobacterium leprae / metabolism*
Oxidation-Reduction
Oxygen / pharmacology
Palmitic Acid
Palmitic Acids / metabolism
Temperature
Grant Support
ID/Acronym/Agency:
AI22492/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Glycolipids; 0/Palmitic Acids; 56-65-5/Adenosine Triphosphate; 57-10-3/Palmitic Acid; 7782-44-7/Oxygen
Comments/Corrections

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