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Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum.
MedLine Citation:
PMID:  21862243     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effect of high hydrostatic pressure application on fetal bovine serum components and the model microorganism (Bovine Viral Diarrheavirus type 1 NADL strain) was studied at 132 and 220MPa pressure for 5min at 25°C. Protein secondary structures were found to be unaffected by an artificial neural network application on the amide I region for both untreated and HHP treated samples. FTIR spectroscopy study of both the HHP-treated and control samples revealed changes in the intensity of some bands in the finger-print region (1500-900cm(-1)) originating mainly from lipids which are thought to result from changes in the lipoprotein structure. The virus strain lost its infectivity completely after 220MPa HHP treatments. These results indicate that HHP can be successfully used for inactivation of pestiviruses while leaving structural and functional properties of serum and serum products unaffected.
Authors:
Cagatay Ceylan; Feride Severcan; Aykut Ozkul; Mete Severcan; Faruk Bozoglu; Nusret Taheri
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-30
Journal Detail:
Title:  Veterinary microbiology     Volume:  -     ISSN:  1873-2542     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Department of Food Engineering, İzmir Institute of Technology, Urla, 35430 İzmir, Turkey.
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