Document Detail

Biophysical investigations into the interactions of endotoxins with bile acids.
MedLine Citation:
PMID:  21954318     Owner:  NLM     Status:  MEDLINE    
The interaction of selected endotoxin preparations (lipid A from Erwinia carotovora and LPS Re and Ra from Salmonella enterica sv. Minnesota strains R595 and R60, respectively) with selected bile acids was investigated biophysically. Endotoxin aggregates were analyzed for their gel-to-liquid crystalline phase behavior, the type of their aggregates, the conformation of particular functional groups, and their Zeta potential in the absence and presence of the bile acids by applying Fourier-transform infrared spectroscopy, differential scanning calorimetry, measurements of the electrophoretic mobility, and synchrotron radiation X-ray scattering. In addition, the ability of the endotoxins to induce cytokines in human mononuclear cells was tested in the absence and presence of varying concentrations of bile acids. The data show that the endotoxin:bile acid interaction is not governed by Coulomb forces, rather a hydrophobic interaction takes place. This leads to an enhanced formation of the inherent cubic aggregate structures of the endotoxins, concomitant with a slight disaggregation, as evidenced by freeze-fracture electron microscopy. Parallel to this, the addition of bile acids increased the bioactivity of lipid A and, to a lower degree, also that of the tested rough mutant LPS at lower concentrations of the endotoxin preparation, a finding similar as reported for the interaction of other agents such as hemoglobin. These data imply that there are general mechanisms that govern the expression of biological activities of endotoxins.
Satoshi Fukuoka; Walter Richter; Jörg Howe; Jörg Andrä; Manfred Rössle; Christian Alexander; Thomas Gutsmann; Klaus Brandenburg
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-27
Journal Detail:
Title:  Innate immunity     Volume:  18     ISSN:  1753-4267     ISO Abbreviation:  Innate Immun     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-16     Completed Date:  2012-07-17     Revised Date:  2012-07-18    
Medline Journal Info:
Nlm Unique ID:  101469670     Medline TA:  Innate Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  307-17     Citation Subset:  IM    
National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.
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MeSH Terms
Bile Acids and Salts / chemistry*
Calorimetry, Differential Scanning
Chenodeoxycholic Acid / chemistry
Cytokines / biosynthesis
Dehydrocholic Acid / chemistry
Deoxycholic Acid / chemistry
Endotoxins / chemistry*
Freeze Fracturing
Lipid A / pharmacology
Lithocholic Acid / chemistry
Monocytes / metabolism
Pectobacterium carotovorum / chemistry
Salmonella enterica / chemistry
Sodium Cholate / chemistry
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cytokines; 0/Endotoxins; 0/Lipid A; 361-09-1/Sodium Cholate; 434-13-9/Lithocholic Acid; 474-25-9/Chenodeoxycholic Acid; 81-23-2/Dehydrocholic Acid; 83-44-3/Deoxycholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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