Document Detail


Biophysical and biochemical properties of CR326 human hepatitis A virus.
MedLine Citation:
PMID:  171955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CR326 human hepatitis A virus purified by isopycnic banding from infected marmoset sera was shown to consist of 27 mmu spherical particles on electron microscopic examination. The particles were identified as hepatitis A virus by tests by infectivity and by specific neutralization of infectivity with convalescent human hepatitis A serum. Also, identical 27 mmu viruses in liver extracts gave specific reactions with hepatitis A antisera when tested by immune electron microscopy. The buoyant density of the virus in CsCl was 1.34 and it was heat (60 C), ether, and acid stable but was destroyed by heat (100 C), formalin (1:4000), and ultraviolet irradiation. Electron microscopic studies of sections of infected marmoset liver showed intracytoplasmic virus particles, usually in vesicles. Presumptive findings for RNA, together with the intracytoplasmic location of the virus, indicated the virus to be of RNA-type. The attributes of the virus indicate it is closely related to the enterovirus family and not to hepatitis B virus.
Authors:
P J Provost; B S Wolanski; W J Miller; O L Ittensohn; W J McAleer; M R Hilleman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of the medical sciences     Volume:  270     ISSN:  0002-9629     ISO Abbreviation:  Am. J. Med. Sci.     Publication Date:    1975 Jul-Aug
Date Detail:
Created Date:  1976-01-08     Completed Date:  1976-01-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370506     Medline TA:  Am J Med Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen-Antibody Reactions
Callitrichinae
Hepatitis A / microbiology*
Hepatitis B Antigens / analysis*
Hepatitis B virus / ultrastructure
Hepatitis Viruses / analysis,  ultrastructure*
Hepatovirus / analysis,  classification,  ultrastructure*
Hot Temperature
Humans
Immune Adherence Reaction
Inclusion Bodies, Viral
Liver / microbiology
Pan troglodytes
RNA, Viral / isolation & purification
Chemical
Reg. No./Substance:
0/Hepatitis B Antigens; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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