| Biophysical and biochemical characterization of the intrinsic fluorescence from neurofibrillary tangles. | |
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MedLine Citation:
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PMID: 15946772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, we developed a novel fluorescent method named intrinsic fluorescence induction that allows direct visualization of neurofibrillary pathology without introducing exogenous chromogens. In the present study, we further characterized the properties of this novel red fluorescence biophysically, biochemically, and neuropathologically. In vitro spectrofluorometry and in situ emission scan show that the intrinsic fluorescence of neurofibrillary tangles has a long emission wavelength peak at 620 nm and a large Stoke's shift of 70 nm. Dephosphorylation of Alzheimer's disease brain sections with alkaline phosphatase or denaturation with guanidine only causes a subtle reduction in the induced fluorescence of neurofibrillary tangles, while hydrofluoric acid or formic acid completely eliminates the fluorescence. Chemical modification of residue serine, but not tyrosine or tryptophan, reduced the intensity of induced fluorescence significantly. The induced fluorophore, thus, has unique properties, and its generation likely depends on the particular conformation of paired helical filaments, which may in turn depend on tau hyperphosphorylation. |
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Authors:
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Guoying Bing; Xuan V Nguyen; Mei Liu; William R Markesbery; Anyang Sun |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2005-06-08 |
Journal Detail:
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Title: Neurobiology of aging Volume: 27 ISSN: 0197-4580 ISO Abbreviation: Neurobiol. Aging Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-08 Completed Date: 2006-07-26 Revised Date: 2012-03-02 |
Medline Journal Info:
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Nlm Unique ID: 8100437 Medline TA: Neurobiol Aging Country: United States |
Other Details:
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Languages: eng Pagination: 823-30 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Neurobiology, University of Kentucky, Lexington, KY 40536-0098, USA. gbing@uky.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Alzheimer Disease / pathology* Animals Brain / drug effects, pathology* Fluorescence* Guanidine / pharmacology Humans Immunohistochemistry Mice Mice, Transgenic Microscopy, Fluorescence / methods Nerve Tissue Proteins / deficiency Neurofibrillary Tangles / drug effects, pathology* Oncogene Proteins / deficiency Phosphorylation Photochemistry* Postmortem Changes Spectrometry, Fluorescence / methods Time Factors Trypsin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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5P50 AG 05144/AG/NIA NIH HHS; NS 39345/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/NREP protein, human; 0/Nerve Tissue Proteins; 0/Oncogene Proteins; 113-00-8/Guanidine; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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