| Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field. | |
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MedLine Citation:
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PMID: 18405105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied biophoton characteristics of Madin-Darby canine kidney (MDCK) cells under the influence of H2O2 by employing a photomultiplier tube (PMT) and a fluorescence microscope. H2O2 was used for producing reactive oxygen species (ROS) in the measurement. Images from a fluorescence microscope show an increase of photon intensity emitted from the sample due to H2O2. By using a PMT we measured quantitative change in biophoton emission with application of H2O2 to the MDCK cell culture, found that the increase of the biophoton is dependent upon the amount of H2O2. The agreement between the results of the PMT and the fluorescence microscope suggests the possibility of quantitative measurement of the influence of ROS on living tissue or cell. In addition we applied a 60 HzAC magnetic field on the cells to investigate the change in reaction between MDCK cell and ROS. It showed that a decay of chemiluminescence intensity has taken a different path following exposure to the magnetic field. As a result, the PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS. |
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Authors:
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B S Cheun; S H Yi; K Y Baik; J K Lim; J S Yoo; H W Shin; K S soh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of environmental biology / Academy of Environmental Biology, India Volume: 28 ISSN: 0254-8704 ISO Abbreviation: J Environ Biol Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2008-04-14 Completed Date: 2008-04-29 Revised Date: 2009-03-09 |
Medline Journal Info:
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Nlm Unique ID: 8300544 Medline TA: J Environ Biol Country: India |
Other Details:
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Languages: eng Pagination: 735-40 Citation Subset: IM |
Affiliation:
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Pacific Yale University Oriental Medicine, 333 East Colorado Boulevera Glendale, CA 91205, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Dogs Hydrogen Peroxide / pharmacology* Magnetics* Photons |
| Chemical | |
Reg. No./Substance:
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7722-84-1/Hydrogen Peroxide |
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