| Biomonitoring of carcinogenic heterocyclic aromatic amines in hair: a validation study. | |
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MedLine Citation:
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PMID: 19588936 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A facile method was established to measure heterocyclic aromatic amines (HAAs) accumulated in human hair and rodent fur. The samples were digested by base hydrolysis, and the liberated HAAs were isolated by tandem solvent/solid-phase extraction. Quantification was done by liquid chromatography/tandem mass spectrometry, using a triple stage quadrupole mass spectrometer in the selected reaction monitoring mode. In a pilot study of 12 human volunteers, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was detected in the hair of six meat-eaters at levels ranging from 290 to 890 pg/g hair. 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-9H-pyrido[2,3-b]indole (AalphaC) were below the limit of quantification (LOQ) (50 pg/g hair) in hair from meat-eaters and six vegetarians. PhIP was detected in the hair from one vegetarian, and at a level just above the LOQ (65 pg/g hair), indicating that PhIP exposure occurs primarily through meat consumption. The levels of PhIP in hair samples from two meat-eaters varied by less than 24% over a 6 month interval, signifying that the exposure to PhIP and its accumulation in hair are relatively constant over time. In a controlled feeding study, female C57BL/6 mice were given these HAAs in their drinking water for 1 month, at six daily dose concentrations ranging from 0 and 0.080 to 800 microg/kg body weight. PhIP was detected in fur of mice at all doses, whereas AalphaC and MeIQx were detected in fur at dosages > or =0.8 mug AalphaC/kg body weight and > or =8 microg MeIQx/kg body weight. There was a strong positive relationship between dosage and each of the HAAs accumulated in fur and their DNA adducts formed in liver and colon (p values < 0.0001); however, the levels of HAA in fur did not correlate to the levels of DNA adducts after adjustment of dose. Thus, hair appears to be a promising tissue with by which we can noninvasively biomonitor the chronic exposure to PhIP, a potential human carcinogen. |
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Authors:
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Erin E Bessette; Isil Yasa; Deborah Dunbar; Lynne R Wilkens; Loic Le Marchand; Robert J Turesky |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Validation Studies |
Journal Detail:
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Title: Chemical research in toxicology Volume: 22 ISSN: 1520-5010 ISO Abbreviation: Chem. Res. Toxicol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-12-01 Completed Date: 2010-05-03 Revised Date: 2013-06-02 |
Medline Journal Info:
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Nlm Unique ID: 8807448 Medline TA: Chem Res Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 1454-63 Citation Subset: IM |
Affiliation:
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Division of Environmental Health Sciences, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, New York 12201, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amines
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analysis*,
metabolism Animals Carcinogens / analysis, toxicity Chromatography, High Pressure Liquid DNA Adducts / analysis Drug Monitoring / methods* Female Hair / chemistry, drug effects Humans Mice Micronucleus Tests / methods Mutagenicity Tests / methods Quinoxalines / toxicity |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA122320-01A1/CA/NCI NIH HHS; R01CA-122320/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Carcinogens; 0/DNA Adducts; 0/Quinoxalines; 63478-55-7/TANDEM (quinoxaline) |
| Comments/Corrections | |
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