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Biomolecular analysis and cancer diagnostics by negative mode probe electrospray ionization.
MedLine Citation:
PMID:  23348832     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We have examined several combinations of solvents and probes with the aim of optimizing the ionization conditions for biomolecules e.g., proteins, peptides and lipids by negative mode probe electrospray ionization mass spectrometry (PESI-MS). With the data presented in this study, negative-mode PESI-MS can be considered as a potential tool for biomolecular analysis and cancer diagnostics because of its simplicity in instrumental configuration. A sharper sampling probe was found to be better for obtaining high quality mass spectra because it can generate stable electrospray without the occurrence of gas breakdown. Although the best conditions may depend on each sample, aqueous organic solvent solutions, especially isopropanol-H(2)O (1/1) with a pH of ≥7, are shown to be preferable for negative-mode PESI-MS, which was successfully applied to colon cancer diagnosis.
Authors:
Mridul Kanti Mandal; Subhrakanti Saha; Kentaro Yoshimura; Yasuo Shida; Sen Takeda; Hiroshi Nonami; Kenzo Hiraoka
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511 Japan. mandal@yamanashi.ac.jp hiraoka@yamanashi.ac.jp.
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