| Biomechanics of the anterior human corneal tissue investigated with Atomic Force Microscopy. | |
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MedLine Citation:
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PMID: 22266511 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose:To investigate the biomechanics of the anterior human corneal stroma using Atomic Force Microscopy (AFM).Methods:AFM measurements were performed in liquid on the anterior stroma of human corneas, after gently removing the epithelium, using a Nanoscope III (Veeco, Sunnyvale, CA, USA) in the force spectroscopy mode. Rectangular silicon cantilevers with tip radius of 10 nm and spring elastic constants of 25- and 33-N/m were used. Each specimen was subjected to increasing loads up to a maximum of 2.7 μN with scan speeds ranging between 3- and 95-μm/s. The anterior stromal hysteresis during the extension-retraction cycle was quantified as a function of the application load and scan rate. The elastic modulus of the anterior stroma was determined by fitting force curve data to the Sneddon model.Results:The anterior stroma exhibited significant viscoelasticity at micrometric level: asymmetry in the curve loading-unloading response with considerable hysteresis dependent both on the application load and scan rate (P<0.01). The mean elastic modulus ranged between 1.14 and 2.63 MPa and was constant over the range of indentation depths between 1.0 and 2.7 μm in the stroma.Conclusions:At microscale level, the mechanical response of the most anterior stroma is complex and non-linear. The microstructure (fibers' packing, number of cross-links, water content) and the combination of elastic (collagen fibers) and viscous (matrix) components of the tissue influence the type of viscoelastic response. Efforts in modeling the biomechanics of human corneal tissue at micrometric level are needed. |
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Authors:
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Marco Lombardo; Giuseppe Lombardo; Giovanni Carbone; Maria P De Santo; Riccardo Barberi; Sebastiano Serrao |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: - ISSN: 1552-5783 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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IRCCS Fondazione G.B. Bietti, Rome, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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