Document Detail

Biomass and aggregation analysis of human embryonic kidney 293 suspension cell cultures by particle size measurement.
MedLine Citation:
PMID:  11027175     Owner:  NLM     Status:  MEDLINE    
A method has been developed to monitor the cell growth of aggregated human embryonic kidney 293 (HEK293) suspension cultures by measuring cumulative particle volume and the particle size distribution. This method employs a particle size analyzer that determines the size of individual particles by detecting their light obscuration (blockage) or scattering. Cell counts derived from the cumulative volume of the cell particle correlate well with manual cell counts from a hemacytometer at different stages of growth. This correlation was further confirmed by quantifying total cellular protein of the samples. Simultaneously, the aggregation state of the samples can also be monitored and mathematically described. Results from this study demonstrate that this simple and reproducible method allows the direct measurement of cumulative cell volume and the degree of cell aggregation, as well as an indirect assessment of cell counts.
Y S Tsao; R G Condon; E J Schaefer; D A Lindsay; Z Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  16     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2000 Sep-Oct
Date Detail:
Created Date:  2000-12-04     Completed Date:  2000-12-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  809-14     Citation Subset:  IM    
Biotechnology Development, Schering-Plough Research Institute, 1011 Morris Avenue, Union, New Jersey 07083, USA.
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MeSH Terms
Cell Aggregation*
Cell Line
Particle Size
Scattering, Radiation

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