| Biomass Conversion Determined via Fluorescent Cellulose Decay Assay. | |
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MedLine Citation:
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PMID: 22608719 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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An example of a rapid microtiter plate assay (fluorescence cellulose decay, FCD) that determines the conversion of cellulose in a washed biomass substrate is reported. The conversion, as verified by HPLC, is shown to correlate to the monitored FCD in the assay. The FCD assay activity correlates to the performance of multicomponent enzyme mixtures and is thus useful for the biomass industry. The development of an optimized setup of the 96-well microtiter plate is described, and is used to test a model that shortens the assay incubation time from 72 to 24h. A step-by-step procedure of the final assay is described. |
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Authors:
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Bente Wischmann; Marianne Toft; Marco Malten; K C McFarland |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 510 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-05-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 19-36 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Novozymes A/S, Bagsværd, Denmark. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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