Document Detail


Biomarkers measured by cytokinesis-block micronucleus cytome assay for evaluating genetic damages induced by polycyclic aromatic hydrocarbons.
MedLine Citation:
PMID:  19540355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Coke oven workers are regularly exposed to polycyclic aromatic hydrocarbons (PAHs) and have a high risk for lung cancer. Limited evidence has demonstrated a direct link between exposure to PAHs and early genetic damage in exposed workers. The cytokinesis-block micronucleus (CBMN) cytome assay is a comprehensive system for measuring DNA damage and cytotoxicity. In the current study, we investigated different chromosomal damage endpoints including micronuclei (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs), in 141 PAH-exposed subjects and 66 unexposed controls. The frequencies of MN, NPBs and NBUDs were all significantly higher in PAH-exposed workers than in controls (2.4-, 5-, and 3-fold, respectively). We further classified the PAH-exposed workers into different PAHs exposure groups based on their work positions on the oven and their urinary 1-hydroxypyrene and found that the frequencies of NPBs and NBUDs increased with the increasing level of both external and internal PAHs exposure levels. Similar trend was not found for MN due to the reduced MN frequency in the highest PAHs exposure group compared with the second highest PAHs exposure group. Using principal component analysis, we confirmed that the frequencies of NPBs and NBUDs are more sensitive to reflect the external or internal levels of PAHs exposure. In PAH-exposed subjects, NPB and NBUD frequencies were influenced by gender and females have lower frequencies of NPB and NBUD. Taken together, our observations indicate that NPBs and NBUDs are more sensitive and reliable biomarkers for genetic damages induced by PAHs and could potentially be used for the biomonitoring of genotoxin-exposed populations.
Authors:
Huawei Duan; Shuguang Leng; Zufei Pan; Yufei Dai; Yong Niu; Chuanfeng Huang; Ping Bin; Yadong Wang; Qingjun Liu; Wen Chen; Yuxin Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-18
Journal Detail:
Title:  Mutation research     Volume:  677     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:    2009 Jun-Jul
Date Detail:
Created Date:  2009-07-17     Completed Date:  2009-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-9     Citation Subset:  IM    
Affiliation:
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Beijing, 100050, PR China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / analysis*
Cytokinesis
DNA Damage*
Female
Humans
Inhalation Exposure
Lymphocytes / ultrastructure
Male
Mutagenicity Tests*
Occupational Exposure / adverse effects*
Polycyclic Hydrocarbons, Aromatic / toxicity*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Polycyclic Hydrocarbons, Aromatic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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