| Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study. | |
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MedLine Citation:
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PMID: 20953827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria. METHODS: Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intra-patient analysis were performed on 17 selected candidates. RESULTS: Seven genes were differentially (p ≤ 0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence. CONCLUSIONS: These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection. Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency. |
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Authors:
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Mourad Assidi; Markus Montag; Katrin Van der Ven; Marc-André Sirard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-16 |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: 28 ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-03-18 Completed Date: 2011-07-08 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: Netherlands |
Other Details:
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Languages: eng Pagination: 173-88 Citation Subset: IM |
Affiliation:
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Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, Ste-Foy, Québec, Canada, G1K 7P4. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cumulus Cells
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cytology*,
metabolism* Embryo Implantation / genetics, physiology Embryonic Development / genetics Female Gene Expression Profiling Genetic Markers Humans Oligonucleotide Array Sequence Analysis Oocytes / cytology, growth & development* Oogenesis / genetics, physiology Ovarian Follicle / cytology, embryology, growth & development Pregnancy Pregnancy Outcome / genetics Sperm Injections, Intracytoplasmic* Triptorelin Pamoate / administration & dosage, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 57773-63-4/Triptorelin Pamoate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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