Document Detail

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study.
MedLine Citation:
PMID:  20953827     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria.
METHODS: Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intra-patient analysis were performed on 17 selected candidates.
RESULTS: Seven genes were differentially (p ≤ 0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence.
CONCLUSIONS: These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection. Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency.
Mourad Assidi; Markus Montag; Katrin Van der Ven; Marc-André Sirard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-16
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  28     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-03-18     Completed Date:  2011-07-08     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  173-88     Citation Subset:  IM    
Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, Ste-Foy, Québec, Canada, G1K 7P4.
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MeSH Terms
Cumulus Cells / cytology*,  metabolism*
Embryo Implantation / genetics,  physiology
Embryonic Development / genetics
Gene Expression Profiling
Genetic Markers
Oligonucleotide Array Sequence Analysis
Oocytes / cytology,  growth & development*
Oogenesis / genetics,  physiology
Ovarian Follicle / cytology,  embryology,  growth & development
Pregnancy Outcome / genetics
Sperm Injections, Intracytoplasmic*
Triptorelin Pamoate / administration & dosage,  pharmacology
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Genetic Markers; 57773-63-4/Triptorelin Pamoate

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