Document Detail


Bioluminescent monitoring of detoxification processes: activity of humic substances in quinone solutions.
MedLine Citation:
PMID:  17716903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study deals with application of bioluminescent assay systems to evaluate the detoxifying effect of humic substances (HS) on the solutions of organic oxidizers - quinones. A series of homologous quinones with different redox characteristics: 1,4-benzoquinone, tetrafluoro-1,4-benzoquinone, methyl-1,4-benzoquinone, tetramethyl-1,4-benzoquinone, and 1,4-naphtoquinone, was used. Bioluminescent bacteria Photobacterium phosphoreum, and NADH:FMN-oxidoreductase-luciferase enzyme system isolated from these bacteria were used as assay systems. The toxicity was compared in the presence and in the absence of HS. Variation of complexity of bioassays (in vivo or in vitro) combined with spectrometric and microscopic methods, provides insight into the process of detoxification in quinone solutions. Two ways of HS effect were studied: the reduction activity of HS and intensification of self-protection of bacterial cells on HS addition.
Authors:
Elena Fedorova; Nadezhda Kudryasheva; Alexander Kuznetsov; Olga Mogil'naya; Devard Stom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-18
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  88     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  131-6     Citation Subset:  IM    
Affiliation:
Institute of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk, Russia.
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MeSH Terms
Descriptor/Qualifier:
FMN Reductase / drug effects*,  metabolism
Humic Substances / analysis*,  toxicity
Luciferases, Bacterial / drug effects*,  metabolism
Luminescent Measurements / methods*
Oxidation-Reduction
Photobacterium / drug effects*,  metabolism,  ultrastructure
Quinones / chemistry,  pharmacology*
Solutions / chemistry
Spectrophotometry
Chemical
Reg. No./Substance:
0/Humic Substances; 0/Luciferases, Bacterial; 0/Quinones; 0/Solutions; EC 1.5.1.29/FMN Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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