Document Detail


Bioluminescent enzymatic rapid assay of water integral toxicity.
MedLine Citation:
PMID:  23151839     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A bioluminescent rapid method was developed to estimate the integral toxicity of natural and wastewater. This method is based on registering the effect of the polluted water sample on the parameters of the bioluminescent reaction catalyzed by the multi-component reagent containing NADH:FMN oxidoreductase, luciferase, and their substrates co-immobilized in a starch carrier. Several ways to increase the method's sensitivity to toxic substances were suggested; conditions were selected to make it possible to determine, with maximum efficiency, the content of toxic substances corresponding to a certain maximum permissible concentration. The sensitivity of soluble and immobilized coupled enzymatic systems to a series of organic pollutants (phenols, quinones, and salts of heavy metals) was compared. It was shown that the reagent is the most sensitive to the effect of phenols and quinones. The method was tested during analysis of the wastewater from a pulp and paper plant and can be used for biotesting in both laboratory and field conditions.
Authors:
E N Esimbekova; A M Kondik; V A Kratasyuk
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-15
Journal Detail:
Title:  Environmental monitoring and assessment     Volume:  -     ISSN:  1573-2959     ISO Abbreviation:  Environ Monit Assess     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8508350     Medline TA:  Environ Monit Assess     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk, 660036, Russia, Esimbekova@yandex.ru.
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