| Bioluminescent assay for total bile acids in serum with use of bacterial luciferase. | |
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MedLine Citation:
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PMID: 6573977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This simple, rapid, sensitive kinetic bioluminescent method for the assay of bile acids in serum involves use of 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) in combination with a new, commercially available NADH Monitoring Reagent (LKB-Wallac) containing a low activity of NADH:FMN-oxidoreductase and a high activity of bacterial luciferase. Interfering dehydrogenases in serum are inactivated in the test tube with trichloroacetic acid before the assay. The standard curve is linear for concentrations of bile acids up to about 300 mumol/L. With a sample volume of 20 microL, the detection limit is about 0.2 mumol/L. The within-run precision (CV) is about 10%, both at high and low concentrations of bile acids in serum. Correlation is good (r = 0.996) between results by this method and an enzymatic method based on spectrophotometry. However, the latter method is considerably less sensitive, and it is less precise at low concentrations of bile acids. |
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Authors:
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I Styrélius; A Thore; I Björkhem |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 29 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1983 Jun |
Date Detail:
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Created Date: 1983-07-15 Completed Date: 1983-07-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1123-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxysteroid Dehydrogenases 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) Bile Acids and Salts / blood* Humans Hydrogen-Ion Concentration Indicators and Reagents Kinetics Luciferases* Luminescent Measurements* Spectrophotometry Trichloroacetic Acid |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Indicators and Reagents; 76-03-9/Trichloroacetic Acid; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.1.1.50/3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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