| Biological variation of free plasma amino acids in healthy individuals. | |
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MedLine Citation:
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PMID: 19929754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Biological variation data for free plasma amino acids are lacking from the more comprehensive databases. Therefore, we determined the intra- and inter-individual components of variation in healthy subjects. These data were used to define desirable goals for imprecision, bias and total error, indices of individuality and reference change values. METHODS: Plasma samples were collected from 11 volunteers at weekly intervals over 5 weeks. Free plasma amino acids were analyzed by reversed-phase HPLC and analytical and biological variation data were derived using ANOVA. RESULTS: Intra-individual coefficients of variation ranged from 9.5% to 46.4%, with lower values among the essential amino acids. The mean inter-individual coefficient of variation was 46.6% and was higher than intra-individual values for all amino acids. Thus, indices of individuality were below 0.8. Reference change values ranged from 30.9% to 128.4% and total error values ranged from 15.2% to 61.0%. CONCLUSIONS: Plasma amino acids exhibit relatively low intra-individual coefficients of variation, with essential amino acids showing tighter homeostatic control. Analytical quality goals can be reasonably achieved with current methods. Reference intervals are of limited value in the detection of unusual results in an individual. Therefore, comparison of serial results by means of the reference change values is recommended. |
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Authors:
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Zoraida Corte; Rafael Venta |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-05 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 99-104 Citation Subset: IM |
Affiliation:
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Servicio de An?lisis Cl?nicos, Hospital San Agust?n, Avil?s, Principado de Asturias, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amino Acids / blood* Analysis of Variance Chromatography, High Pressure Liquid Female Humans Male Middle Aged Reference Values |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
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