| Biological nanofactories facilitate spatially selective capture and manipulation of quorum sensing bacteria in a bioMEMS device. | |
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MedLine Citation:
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PMID: 20390130 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The emergence of bacteria that evade antibiotics has accelerated research on alternative approaches that do not target cell viability. One such approach targets cell-cell communication networks mediated by small molecule signaling. In this report, we assemble biological nanofactories within a bioMEMS device to capture and manipulate the behavior of quorum sensing (QS) bacteria as a step toward modifying small molecule signaling. Biological nanofactories are bio-inspired nanoscale constructs which can include modules with different functionalities, such as cell targeting, molecular sensing, product synthesis, and ultimately self-destruction. The biological nanofactories reported here consist of targeting, sensing, synthesis and, importantly, assembly modules. A bacteria-specific antibody constitutes the targeting module while a genetically engineered fusion protein contains the sensing, synthesis and assembly modules. The nanofactories are assembled on chitosan electrodeposited within a microchannel of the bioMEMS device; they capture QS bacteria in a spatially selective manner and locally synthesize and deliver the "universal" small signaling molecule autoinducer-2 (AI-2) at the captured cell surface. The nanofactory based AI-2 delivery is demonstrated to alter the progression of the native AI-2 based QS response of the captured bacteria. Prospects are envisioned for utilizing our technique as a test-bed for understanding the AI-2 based QS response of bacteria as a means for developing the next generation of antimicrobials. |
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Authors:
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Rohan Fernandes; Xiaolong Luo; Chen-Yu Tsao; Gregory F Payne; Reza Ghodssi; Gary W Rubloff; William E Bentley |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-02-16 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 1128-34 Citation Subset: IM |
Affiliation:
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Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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instrumentation Cell Culture Techniques / instrumentation* Cell Separation / instrumentation* Equipment Design Equipment Failure Analysis Escherichia coli / physiology* Micro-Electrical-Mechanical Systems / instrumentation* Microfluidic Analytical Techniques / instrumentation* Micromanipulation / instrumentation* Nanotechnology / instrumentation Quorum Sensing / physiology* Reproducibility of Results Sensitivity and Specificity |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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