Document Detail

Biological nanofactories facilitate spatially selective capture and manipulation of quorum sensing bacteria in a bioMEMS device.
MedLine Citation:
PMID:  20390130     Owner:  NLM     Status:  MEDLINE    
The emergence of bacteria that evade antibiotics has accelerated research on alternative approaches that do not target cell viability. One such approach targets cell-cell communication networks mediated by small molecule signaling. In this report, we assemble biological nanofactories within a bioMEMS device to capture and manipulate the behavior of quorum sensing (QS) bacteria as a step toward modifying small molecule signaling. Biological nanofactories are bio-inspired nanoscale constructs which can include modules with different functionalities, such as cell targeting, molecular sensing, product synthesis, and ultimately self-destruction. The biological nanofactories reported here consist of targeting, sensing, synthesis and, importantly, assembly modules. A bacteria-specific antibody constitutes the targeting module while a genetically engineered fusion protein contains the sensing, synthesis and assembly modules. The nanofactories are assembled on chitosan electrodeposited within a microchannel of the bioMEMS device; they capture QS bacteria in a spatially selective manner and locally synthesize and deliver the "universal" small signaling molecule autoinducer-2 (AI-2) at the captured cell surface. The nanofactory based AI-2 delivery is demonstrated to alter the progression of the native AI-2 based QS response of the captured bacteria. Prospects are envisioned for utilizing our technique as a test-bed for understanding the AI-2 based QS response of bacteria as a means for developing the next generation of antimicrobials.
Rohan Fernandes; Xiaolong Luo; Chen-Yu Tsao; Gregory F Payne; Reza Ghodssi; Gary W Rubloff; William E Bentley
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-02-16
Journal Detail:
Title:  Lab on a chip     Volume:  10     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-14     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1128-34     Citation Subset:  IM    
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
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MeSH Terms
Biological Assay / instrumentation
Cell Culture Techniques / instrumentation*
Cell Separation / instrumentation*
Equipment Design
Equipment Failure Analysis
Escherichia coli / physiology*
Micro-Electrical-Mechanical Systems / instrumentation*
Microfluidic Analytical Techniques / instrumentation*
Micromanipulation / instrumentation*
Nanotechnology / instrumentation
Quorum Sensing / physiology*
Reproducibility of Results
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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