Document Detail

Biological heterogeneity of putative bladder cancer stem-like cell populations from human bladder transitional cell carcinoma samples.
MedLine Citation:
PMID:  19961489     Owner:  NLM     Status:  MEDLINE    
Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Emerging evidence has suggested that the capability of a tumor to grow and propagate is dependent on a small subset of cells, the cancer stem-like cells (CSCs) or tumor initiating cells. We report on the isolation and biological characterization of putative bladder CSC populations from primary TCCs. Isolated cells were induced to proliferate in stem cell culture conditions (serum-free medium containing mitogenic growth factors). The proliferating cells formed spheroids (urospheres) and their abilities for extensive proliferation and self-renewal were assayed. Their positivity for several stem cell markers (CD133, Oct-3/4, nestin, and cytokeratins) was also assessed by immunofluorescence tests and they could have the potential to differentiate in the presence of serum. In stem cell culture conditions they gradually showed loss of proliferation, adherence to the substrate, and morphological changes, which might reflect their progressive acquisition of differentiative capacity and loss of self-renewal ability. To evaluate if effective cell selection occurred after isolation, conventional cytogenetic studies on fresh chromosome spreads immediately after isolation and after culture were carried out. In addition, a molecular cytogenetic study by UroVysion assay was carried out on paraffin-embedded tissue sections and on fresh and after culture nuclei preparations. The data collected indicated important karyotype changes and a positive selection for hypo- or near-diploid cells, losing the complexity present in fresh tumors.
Angela Bentivegna; Donatella Conconi; Elena Panzeri; Elena Sala; Giorgio Bovo; Paolo Vigan?; Silvia Brunelli; Mario Bossi; Giovanni Tredici; Guido Strada; Leda Dalpr?
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-27
Journal Detail:
Title:  Cancer science     Volume:  101     ISSN:  1349-7006     ISO Abbreviation:  Cancer Sci.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-23     Completed Date:  2010-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  416-24     Citation Subset:  IM    
Dipartimento di Neuroscienze e Tecnologie Biomediche, Universit? degli Studi di Milano-Bicocca, Monza, Italy.
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MeSH Terms
Aged, 80 and over
Antigens, CD / analysis
Carcinoma, Transitional Cell / genetics,  pathology*
Cell Differentiation
Chromosome Aberrations
Glycoproteins / analysis
Middle Aged
Neoplastic Stem Cells / pathology*
Peptides / analysis
Urinary Bladder Neoplasms / genetics,  pathology*
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides

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