Document Detail


Biological evidence of genetic exchange in Entamoeba histolytica.
MedLine Citation:
PMID:  2908287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The demonstration of a new zymodeme of Entamoeba histolytica produced in culture from cloned isolates suggested possible genetic exchange in this parasite. We have attempted to substantiate that finding by using rats as biological hosts. Clones were made from 3 separate isolates of E. histolytica, each established in culture from liver pus or faeces. After enzyme characterization these clones, of zymodemes II, XIV and XIX, were paired in each of the 3 possible combinations and the mixtures injected into the caecum of rats. Clones of new or hybrid zymodemes were produced as well as the original parents, with one exception, the mixture of XIV and XIX, from which only one of the parents was recovered. The hybrids produced included zymodeme XX, observed previously, and zymodeme XI, a naturally occurring zymodeme that has in the past been recovered only from subjects with invasive amoebiasis.
Authors:
P G Sargeaunt; T F Jackson; S R Wiffen; R Bhojnani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transactions of the Royal Society of Tropical Medicine and Hygiene     Volume:  82     ISSN:  0035-9203     ISO Abbreviation:  Trans. R. Soc. Trop. Med. Hyg.     Publication Date:  1988  
Date Detail:
Created Date:  1989-10-25     Completed Date:  1989-10-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7506129     Medline TA:  Trans R Soc Trop Med Hyg     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  862-7     Citation Subset:  IM    
Affiliation:
Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Clone Cells
Electrophoresis
Entamoeba histolytica / enzymology,  genetics*
Mutation
Rats
Rats, Inbred Strains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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