Document Detail

Biological evaluation of solid freeformed, hard tissue scaffolds for orthopedic applications.
MedLine Citation:
PMID:  20740471     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Hydroxyapatite (HA) lattices were made by extrusion freeforming, a rapid prototyping process, and sintered to produce hard tissue scaffolds for bone regeneration. These highly reticulated lattice structures can be built directly from a computer design file which decides and controls their macroscopic shape, pore structure and size distribution. They are therefore defect-specific and show potential in tissue engineering for non-load bearing sites.
METHODS: Using a commercial human osteoblast-like cell line (HOS TE 85), biocompatibility was evaluated in an in vitro study. A high level of cell adhesion was evident by scanning electron microscopy on both convex and concave surfaces and the cell attachment was revealed at different depths into the scaffold. An AlamarBlue(R) assay was carried out to assess cell proliferation, which was further confirmed by quantifying total DNA concentration and total protein content.
RESULTS: The cell proliferation was significant and the pattern was comparable to that of the tissue culture control, ThermanoxTM. ALP activity and osteocalcin were quantified to evaluate the extent of cell differentiation, which confirmed the retention of the phenotype for the period studied. Mineralization of the matrix was determined via formation of nodules.
CONCLUSIONS: HA scaffolds are non-toxic, able to maintain cell viability and support cell growth, proliferation, differentiation, and nodule formation.
H M Thushari U Herath; Lucy Di Silvio; Julian R G Evans
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied biomaterials & biomechanics : JABB     Volume:  8     ISSN:  1722-6899     ISO Abbreviation:  J Appl Biomater Biomech     Publication Date:    2010 May-Aug
Date Detail:
Created Date:  2010-08-26     Completed Date:  2010-12-10     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  101180437     Medline TA:  J Appl Biomater Biomech     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  89-96     Citation Subset:  IM    
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MeSH Terms
Bone Regeneration*
Bone Substitutes / chemistry*
Calcification, Physiologic
Cell Differentiation
Cell Line, Tumor
Cell Proliferation
DNA / analysis,  biosynthesis
Durapatite / chemistry*
Materials Testing / methods*
Osteocalcin / analysis,  biosynthesis
Tissue Engineering / methods*
Tissue Scaffolds*
Reg. No./Substance:
0/Bone Substitutes; 104982-03-8/Osteocalcin; 9007-49-2/DNA; 91D9GV0Z28/Durapatite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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