| Biological evaluation of Mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines. | |
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MedLine Citation:
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PMID: 2365461 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Temperature-sensitive mutants (TSMs) of Mycoplasma pulmonis were produced by treating the wild-type strain with N-methyl-N'-nitro-N-nitrosoguanidine. Three TSMs were selected at 38 degrees C, as a restrictive temperature, and at 34 degrees C, as a permissive temperature. Two TSMs, UTCMI and UTCMII, were proven to be nonpathogenic but immunogenic. In addition, they did not induce pneumonia, tracheitis, or tympanitis but did induce mild rhinitis. They were stable after 10 passages in vitro and in vivo. They elicited excellent antibody production and cell-mediated immunity in vaccinated rats. They also were not mitogenic to rat lymphocytes. Rats immunized intranasally with these TSMs were significantly protected against challenge with wild-type organisms. These mutants were morphologically and serologically indistinguishable from the wild-type organisms. The growth characteristics and antibiotic sensitivities were similar to those of wild-type organisms, except that they grew only at 34 degrees C. In contrast to wild-type organisms, they did not bind to or lyse sheep erythrocytes. Thus, these TSMs may qualify as a vaccine to prevent M. pulmonis infection in rats. |
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Authors:
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W C Lai; M Bennett; Y S Lu; S P Pakes |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 58 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-08-13 Completed Date: 1990-08-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2289-96 Citation Subset: IM |
Affiliation:
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Division of Comparative Medicine, University of Texas Southwestern Medical Center, Dallas 75235. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Bacterial / biosynthesis Bacterial Vaccines / immunology* Female Lung / microbiology Male Mutation Mycoplasma / genetics, immunology, pathogenicity Mycoplasma Infections / prevention & control*, veterinary Nasal Cavity / microbiology Rats Rats, Inbred Lew Rodent Diseases / prevention & control Temperature Vaccines, Attenuated / immunology |
| Grant Support | |
ID/Acronym/Agency:
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RR00890/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Bacterial Vaccines; 0/Vaccines, Attenuated |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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