| Biological and environmental factors affecting ultrasound-induced hemolysis in vitro: 5. Temperature. | |
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MedLine Citation:
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PMID: 16785011 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This research project tested the hypothesis that cold-equilibrated (approximately 0 degrees C) human erythrocytes in vitro in the presence of an ultrasound contrast agent (Albunex) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37 degrees C) human erythrocytes in vitro because of a temperature-related transition in membrane fluidity leading to increased fragility. First, it was shown that cold-equilibrated erythrocytes are more susceptible to mechanically induced hemolysis than physiologically equilibrated erythrocytes. Second, when adjustments were made for (1) temperature-dependent efficiencies of a 1-MHz transducer (200 micros pulse length, 20 ms interpulse interval, 30 s exposure duration) such that when cold or physiological temperatures were employed, there were equivalent acoustic outputs in terms of peak negative pressure (MPa P-) and (2) comparable viscosities of the 0 and 37 degrees C blood plasmas, the cold (approximately 0 degrees C) erythrocytes displayed substantially greater amounts of ultrasound-induced hemolysis than the physiological (37 degrees C) erythrocytes. The data supported the hypothesis. |
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Authors:
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Morton W Miller; Charles C Church; Cecille Labuda; Salvatore Mazza; Jason Raymond |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Ultrasound in medicine & biology Volume: 32 ISSN: 0301-5629 ISO Abbreviation: Ultrasound Med Biol Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-20 Completed Date: 2006-08-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0410553 Medline TA: Ultrasound Med Biol Country: England |
Other Details:
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Languages: eng Pagination: 893-904 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. Morton_Miller@urmc.rochester.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Viscosity Erythrocyte Membrane / physiology Hemolysis* Humans Oxygen / blood Partial Pressure Temperature Transducers Ultrasonography* |
| Grant Support | |
ID/Acronym/Agency:
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R37CA39230-28/CA/NCI NIH HHS; R37EB00213-28/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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