| Biological effects of naturally occurring and man-made fibres: in vitro cytotoxicity and mutagenesis in mammalian cells. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 10188871 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cytotoxicity and mutagenicity of tremolite, erionite and the man-made ceramic (RCF-1) fibre were studied using the human-hamster hybrid A(L) cells. Results from these fibres were compared with those of UICC Rhodesian chrysotile fibres. The A(L) cell mutation assay, based on the S1 gene marker located on human chromosome 11, the only human chromosome contained in the hybrid cell, has been shown to be more sensitive than conventional assays in detecting deletion mutations. Tremolite, erionite and RCF-1 fibres were significantly less cytotoxic to A(L) cells than chrysotile. Mutagenesis studies at the HPRT locus revealed no significant mutant yield with any of these fibres. In contrast, both erionite and tremolite induced dose-dependent S1- mutations in fibre-exposed cells, with the former inducing a significantly higher mutant yield than the latter fibre type. On the other hand, RCF-1 fibres were largely non-mutagenic. At equitoxic doses (cell survival at approximately 0.7), erionite was found to be the most potent mutagen among the three fibres tested and at a level comparable to that of chrysotile fibres. These results indicate that RCF-1 fibres are non-genotoxic under the conditions used in the studies and suggest that the high mesothelioma incidence previously observed in hamster may either be a result of selective sensitivity of hamster pleura to fibre-induced chronic irritation or as a result of prolonged fibre treatment. Furthermore, the relatively high mutagenic potential for erionite is consistent with its documented carcinogenicity. |
| | |
Authors:
|
R Okayasu; L Wu; T K Hei |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: British journal of cancer Volume: 79 ISSN: 0007-0920 ISO Abbreviation: Br. J. Cancer Publication Date: 1999 Mar |
Date Detail:
|
Created Date: 1999-04-15 Completed Date: 1999-04-15 Revised Date: 2010-09-10 |
Medline Journal Info:
|
Nlm Unique ID: 0370635 Medline TA: Br J Cancer Country: SCOTLAND |
Other Details:
|
Languages: eng Pagination: 1319-24 Citation Subset: IM |
Affiliation:
|
Department of Radiation Oncology, University of Texas Medical Branch, Galveston 77555, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Asbestos, Amphibole / toxicity* Asbestos, Serpentine / toxicity* Cell Line Cell Survival Ceramics / toxicity* Cricetinae Humans Hybrid Cells Kaolin / toxicity* Mineral Fibers / toxicity* Mutagenicity Tests Zeolites / toxicity* |
| Grant Support | |
ID/Acronym/Agency:
|
CA 71438/CA/NCI NIH HHS; ES 05876/ES/NIEHS NIH HHS; ES06831/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Asbestos, Amphibole; 0/Asbestos, Serpentine; 0/Ceramics; 0/Mineral Fibers; 0/RCF-1 fiber; 12510-42-8/erionite; 1318-02-1/Zeolites; 1332-58-7/Kaolin; 14567-73-8/tremolite |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): Br J Cancer ISSN: 0007-0920 ISSN: 1532-1827 Publisher: Nature Publishing Group |
Article Information Download PDF  Copyright 1999, Cancer Research Campaign Received Day: 15 Month: 10 Year: 1997 Revision Received Day: 16 Month: 09 Year: 1998 Accepted Day: 22 Month: 09 Year: 1998 Print publication date: Month: 03 Year: 1999 Volume: 79 Issue: 9/10 First Page: 1319 Last Page: 1324 ID: 2362703 Publisher Item Identifier: 6690213 DOI: 10.1038/sj.bjc.6690213 PubMed Id: 10188871 |
| Biological effects of naturally occurring and man-made fibres: in vitro cytotoxicity and mutagenesis in mammalian cells | |
| R Okayasu1 | |
| L Wu2 | |
| T K Hei2 | |
|
1Department of Radiation Oncology, Biology Division, Galveston, TX, 77555, USA |
|
|
2Center for Radiological Research, College of Physicians and Surgeons of Columbia University, New York, NY, 10032, USA |
|
| *Author for correspondence: |
|
Article Categories:
Keywords: chrysotile, tremolite, erionite, RCF-1, mutation, A L cells. |
|
Previous Document: Long-term survival after extended surgical resection of melanoma metastasis and immunotherapy.
Next Document: Induction of apoptosis in myeloid leukaemic cells by ribozymes targeted against AML1/MTG8.