Document Detail


Biological effects on granulosa cells of hydroxylated and methylated resveratrol analogues.
MedLine Citation:
PMID:  20140899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several resveratrol analogues have been designed to improve bioactivity: among these polymethoxystilbenes appear to be particularly promising. The present study was set up to investigate the biological functions of polymethoxystilbenes 2 and 3, recently found in our lab as antiangiogenic agents, on a well-defined swine granulosa cell model. Proliferative activity and effects on steroidogenesis were evaluated, as well as the effect on granulosa cell vascular endothelial growth factor (VEGF) production, since these cells in basic conditions synthesize the main proangiogenic peptide. Moreover, we considered the effect of these two resveratrol analogues on granulosa cell redox status. Analogue 3 inhibited granulosa cell growth, while it stimulated steroidogenesis. A similar effect was displayed by 2 on estradiol 17beta production and cell proliferation at the highest concentration tested. On the other hand, at the same dosage 2 decreased progesterone levels. Both analogues inhibited VEGF output. Granulosa cell redox status was unaffected by resveratrol analogue 2 while the highest concentration of 3 stimulated free radicals generation and scavenging enzyme activities. The overall results indicate that analogue 3 is the more powerful compound, thus suggesting that a slight modification in the structure markedly increases effectiveness. These data could be useful to develop more active resveratrol analogues for therapeutic use.
Authors:
Giuseppina Basini; Corrado Tringali; Laura Baioni; Simona Bussolati; Carmela Spatafora; Francesca Grasselli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54 Suppl 2     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-29     Completed Date:  2010-12-08     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  S236-43     Citation Subset:  IM    
Affiliation:
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti-Sezione di Fisiologia, Parma, Italy. basini@unipr.it
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MeSH Terms
Descriptor/Qualifier:
Angiogenesis Inhibitors / chemistry*,  pharmacology*
Animals
Cell Proliferation / drug effects
Cell Survival
Cells, Cultured
Drug Evaluation, Preclinical
Estradiol / metabolism
Female
Granulosa Cells / drug effects*,  enzymology,  metabolism
Hydrogen Peroxide / metabolism
Hydroxylation
Methylation
Osmolar Concentration
Ovarian Follicle / cytology
Oxidoreductases / metabolism
Progesterone / metabolism
Stilbenes / chemistry*,  pharmacology*
Superoxides / metabolism
Sus scrofa
Vascular Endothelial Growth Factors / metabolism
Chemical
Reg. No./Substance:
0/Angiogenesis Inhibitors; 0/Stilbenes; 0/Vascular Endothelial Growth Factors; 11062-77-4/Superoxides; 50-28-2/Estradiol; 57-83-0/Progesterone; 7722-84-1/Hydrogen Peroxide; EC 1.-/Oxidoreductases; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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