Document Detail


Biological dynamics and distribution of 3-hydroxy fatty acids in the yeast Dipodascopsis uninucleata as investigated by immunofluorescence microscopy. Evidence for a putative regulatory role in the sexual reproductive cycle.
MedLine Citation:
PMID:  9637254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dipodascopsis uninucleata has been recently shown to produce 3-hydroxy polyenoic fatty acids from several exogenous polyenoic fatty acids. In order to examine whether endogenous 3-hydroxy fatty acids (3-OH-FA) may be implicated in the developmental biology of this yeast, we mapped by immunofluorescence microscopy their occurrence in fixed cells with or without cell walls using an antibody raised against 3R-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (3R-HETE), the biotransformation product from arachidonic acid (AA). This antibody turned out to cross-react with other 3-OH-FA. 3-OH-FA were detected in situ in gametangia, asci, as well as between released ascospores, and proved to be associated with the sexual reproductive stage of the life cycle of the yeast. Acetylsalicylic acid (1 mM), which is known to suppress the formation of 3-OH-FA from exogenous polyenoic fatty acids, inhibited the occurrence of immunoreactive material as well as the sexual phase of the life cycle suggesting a prominent regulatory role of 3-OH-FA for the latter.
Authors:
J L Kock; P Venter; D Linke; T Schewe; S Nigam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  427     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-06     Completed Date:  1998-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  345-8     Citation Subset:  IM    
Affiliation:
Department of Gynecology, University Medical Centre Benjamin Franklin, Free University Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Ascomycota / metabolism*,  physiology,  ultrastructure
Cross Reactions
Fatty Acids, Unsaturated / immunology,  metabolism*,  physiology
Hydroxyeicosatetraenoic Acids / immunology
Microscopy, Fluorescence
Rabbits
Reproduction
Chemical
Reg. No./Substance:
0/Antibodies; 0/Fatty Acids, Unsaturated; 0/Hydroxyeicosatetraenoic Acids; 135295-02-2/3-hydroxy-5,8,11,14-eicosatetraenoic acid

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