| Biological disposition of boldine: in vitro and in vivo studies. | |
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MedLine Citation:
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PMID: 10861968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Boldine is a natural compound with well-established free radical scavenger and hepatoprotective properties. The further exploration of its actual therapeutic potential as an antioxidant is, however, partially limited by the absence of knowledge on its pharmacokinetics. In the present studies, we provide information on the in vitro and in vivo biological disposition of boldine. The addition of 200 microM boldine to an isolated rat hepatocyte suspension was followed by a time-dependent (0-60 min) disappearance of boldine from the extracellular medium. This decline was associated with an early (first 2 min) and swift accumulation (1600 microM) of boldine within the cells. Although the intracellular concentration of boldine diminished, boldine was always found to occur within the cells at concentrations substantially higher than those initially added to the preparation. Boldine was also concentration-dependently removed from the extracellular medium by isolated rat livers portally perfused with the antioxidant. In vivo studies, conducted in rats, revealed that following either its oral or its intravenous administration, plasma boldine concentrations declined rapidly and according to an apparently first order type of kinetics. After its oral administration (50 or 75 mg/kg), boldine was rapidly (within 30 min) absorbed and preferentially concentrated in the liver, with substantially lower concentrations being found in the brain and heart. Maximal hepatic concentrations of boldine were found to be equal to or greater than those needed to afford antioxidant and hepatoprotective effects in vitro. |
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Authors:
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I Jiménez; H Speisky |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: 14 ISSN: 0951-418X ISO Abbreviation: Phytother Res Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-08-22 Completed Date: 2000-08-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 254-60 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Ltd. |
Affiliation:
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Laboratory of Lipids and Antioxidants, Nutrition and Food Technology Institute, University of Chile, Santiago, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aporphines / blood, pharmacokinetics* Liver / cytology, metabolism Male Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Aporphines; 476-70-0/boldine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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