Document Detail


Biological characterization of cells derived from a human breast carcinoma. I. Some characteristics of cells cultivated in vitro prior to and after transplantation into nude mice.
MedLine Citation:
PMID:  7082129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single cells from a patient (B. E., 65 years) have been isolated by collagenase treatment and cultivated in vitro. The human tumour of the mammary gland showed predominantly simple undifferentiated and also tubular structures. The 5th in vitro passage of these cells was characterized by DNA-distribution pattern, cell doubling time, chromosome number, ultrastructure, hormone and drug sensitivity. Cells of the 5th in vitro passage were i.p. transplanted into nude mice. The characteristics of in vitro cultivated cells (5th in vitro passage) were compared with both ascitic cells (1703/A) and cells of solid tumour material (1703/S) grown in nude mice for several passages (0, 10, 21). DNA-distribution patterns, chromosome numbers and cell doubling times are in good correlation. The number of polyploid cells is increased in ascitic cells. These malignant cells are best able to proliferate in vitro after transplantation into nude mice. Ultrastructure examination of the 21st passage has shown similarity between cultured cells, ascitic cells and cells of solid tumour material grown in nude mice. Virus particles could be observed in cells of solid only. Estrogen binding could be observed in the original tumour material only. All other cell or tissue preparations contained no receptors. Drug sensitivity was changed in the case of Vinblastin, Daunoblastin and Sarkolysin treatment of cells of solid tumour material more than in ascitic cells grown in nude mice. The environment dependence of cells and biological differences between in vivo-in vitro tumours and human neoplasms has to be taken into account when using cells as in vivo or in vitro model systems in experimental and clinical cancer research.
Authors:
E Nissen; W Arnold; H Weiss; M Görlich; B Elbe; H Naundorf; S Tanneberger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archiv für Geschwulstforschung     Volume:  52     ISSN:  0003-911X     ISO Abbreviation:  Arch. Geschwulstforsch.     Publication Date:  1982  
Date Detail:
Created Date:  1982-07-22     Completed Date:  1982-07-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372411     Medline TA:  Arch Geschwulstforsch     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  17-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Animals
Antineoplastic Agents / pharmacology,  therapeutic use
Breast Neoplasms / analysis,  drug therapy,  genetics,  pathology*,  ultrastructure
Cell Division
Cells, Cultured
Chromosomes, Human
DNA, Neoplasm / analysis
Drug Resistance
Female
Hormones / pharmacology
Humans
Mice
Mice, Nude
Neoplasm Transplantation
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/DNA, Neoplasm; 0/Hormones

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