| Biological characterisation of vascular grafts cultured in a bioreactor. | |
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MedLine Citation:
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PMID: 16343614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the development is described of a tissue-engineered construct mimicking the structure of a natural blood vessel. Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin. Under these dynamic culture conditions, average wall shear rate, systolic and diastolic pressures and pressure wave-forms comparable to conditions in the human carotid artery were obtained. Culturing of SMC in tubular scaffolds under dynamic conditions resulted in enhanced tissue formation compared to static conditions. Higher SMC numbers, a more homogeneous distribution of SMC throughout the scaffolds and higher collagen mRNA expression levels were found when cells were cultured under dynamic compared to static conditions. mRNA expression levels of markers of proliferation and apoptosis showed that the higher cell numbers in the scaffolds cultured under dynamic conditions can be explained by increased cell proliferation but not by decreased apoptosis. Glucose consumption and lactate formation by the cells showed that cell metabolism was more aerobic under dynamic compared to static conditions. Lining of the dynamically cultured constructs with a luminal monolayer of endothelial cells might result in vessels suitable for in vivo applications. |
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Authors:
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Paula Engbers-Buijtenhuijs; Laura Buttafoco; Andre A Poot; Piet J Dijkstra; Rob A I de Vos; Lotus M Th Sterk; Rob H Geelkerken; Istvan Vermes; Jan Feijen |
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Publication Detail:
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Type: Journal Article Date: 2005-12-15 |
Journal Detail:
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Title: Biomaterials Volume: 27 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2005-12-26 Completed Date: 2006-04-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 2390-7 Citation Subset: IM |
Affiliation:
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Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology and Institute for Biomedical Technology (BMTI), University of Twente, Enschede, P.O. Box 217, 7500 AE, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials Biomechanics Bioreactors* Blood Vessel Prosthesis* Carotid Arteries / anatomy & histology, physiology Cells, Cultured Collagen Type I / genetics Cross-Linking Reagents Cyclin E / genetics Elastin / genetics Gene Expression Humans Materials Testing Microscopy, Electron, Scanning Muscle, Smooth, Vascular / cytology, metabolism Pulsatile Flow RNA, Messenger / genetics, metabolism Tissue Engineering* / instrumentation, methods Transglutaminases / genetics |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Collagen Type I; 0/Cross-Linking Reagents; 0/Cyclin E; 0/RNA, Messenger; 9007-58-3/Elastin; EC 2.3.2.13/Transglutaminases; EC 2.3.2.13/tissue transglutaminase 2, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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