Document Detail

Biological characterisation of vascular grafts cultured in a bioreactor.
MedLine Citation:
PMID:  16343614     Owner:  NLM     Status:  MEDLINE    
In this study, the development is described of a tissue-engineered construct mimicking the structure of a natural blood vessel. Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin. Under these dynamic culture conditions, average wall shear rate, systolic and diastolic pressures and pressure wave-forms comparable to conditions in the human carotid artery were obtained. Culturing of SMC in tubular scaffolds under dynamic conditions resulted in enhanced tissue formation compared to static conditions. Higher SMC numbers, a more homogeneous distribution of SMC throughout the scaffolds and higher collagen mRNA expression levels were found when cells were cultured under dynamic compared to static conditions. mRNA expression levels of markers of proliferation and apoptosis showed that the higher cell numbers in the scaffolds cultured under dynamic conditions can be explained by increased cell proliferation but not by decreased apoptosis. Glucose consumption and lactate formation by the cells showed that cell metabolism was more aerobic under dynamic compared to static conditions. Lining of the dynamically cultured constructs with a luminal monolayer of endothelial cells might result in vessels suitable for in vivo applications.
Paula Engbers-Buijtenhuijs; Laura Buttafoco; Andre A Poot; Piet J Dijkstra; Rob A I de Vos; Lotus M Th Sterk; Rob H Geelkerken; Istvan Vermes; Jan Feijen
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Publication Detail:
Type:  Journal Article     Date:  2005-12-15
Journal Detail:
Title:  Biomaterials     Volume:  27     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2005-12-26     Completed Date:  2006-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  2390-7     Citation Subset:  IM    
Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology and Institute for Biomedical Technology (BMTI), University of Twente, Enschede, P.O. Box 217, 7500 AE, The Netherlands.
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MeSH Terms
Biocompatible Materials
Blood Vessel Prosthesis*
Carotid Arteries / anatomy & histology,  physiology
Cells, Cultured
Collagen Type I / genetics
Cross-Linking Reagents
Cyclin E / genetics
Elastin / genetics
Gene Expression
Materials Testing
Microscopy, Electron, Scanning
Muscle, Smooth, Vascular / cytology,  metabolism
Pulsatile Flow
RNA, Messenger / genetics,  metabolism
Tissue Engineering* / instrumentation,  methods
Transglutaminases / genetics
Reg. No./Substance:
0/Biocompatible Materials; 0/Collagen Type I; 0/Cross-Linking Reagents; 0/Cyclin E; 0/RNA, Messenger; 9007-58-3/Elastin; EC; EC transglutaminase 2, human

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