Document Detail

Biological, biochemical and physicochemical evidence for the existence of the polyadenylate - polyuridylate - poly 2'-fluoro-2'-deoxyuridylate triple-stranded complex.
MedLine Citation:
PMID:  133766     Owner:  NLM     Status:  MEDLINE    
The interferon-inducing activity of the double-stranded complex poly(A) - poly(U) in primary rabbit kidney cell cultures is reduced when the cells are treated with poly(dUfl) either 1 h before, simultaneously with, or 1 h after the exposure to the double-stranded complex. It has been demonstrated in experiments involving sensitivity to hydrolysis by RNAase, UV absorbance-mixing curves, and UV absorbance-temperature profiles that this phenomenon is due to the formation of the triple-stranded complex poly(A) - poly(U) - poly(dUfl). The latter complex seems to be the principal product of interactions in the following systems: poly(A) - poly(U) + poly(dUfl); poly(A) - poly(dUfl) + poly(U); and poly(A) + poly(U) + poly (dUfl).
E De Clercq; B Janik; R G Sommer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemico-biological interactions     Volume:  14     ISSN:  0009-2797     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-10-20     Completed Date:  1976-10-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-25     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Floxuridine / pharmacology
Interferons / biosynthesis*
Kidney / drug effects,  metabolism
Nucleic Acid Hybridization
Poly A / pharmacology
Poly U / pharmacology
Polynucleotides / pharmacology*
Ribonucleases / metabolism
Reg. No./Substance:
0/Polynucleotides; 24937-83-5/Poly A; 27416-86-0/Poly U; 50-91-9/Floxuridine; 9008-11-1/Interferons; EC 3.1.-/Ribonucleases

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