| Biological, biochemical and physicochemical evidence for the existence of the polyadenylate - polyuridylate - poly 2'-fluoro-2'-deoxyuridylate triple-stranded complex. | |
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MedLine Citation:
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PMID: 133766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interferon-inducing activity of the double-stranded complex poly(A) - poly(U) in primary rabbit kidney cell cultures is reduced when the cells are treated with poly(dUfl) either 1 h before, simultaneously with, or 1 h after the exposure to the double-stranded complex. It has been demonstrated in experiments involving sensitivity to hydrolysis by RNAase, UV absorbance-mixing curves, and UV absorbance-temperature profiles that this phenomenon is due to the formation of the triple-stranded complex poly(A) - poly(U) - poly(dUfl). The latter complex seems to be the principal product of interactions in the following systems: poly(A) - poly(U) + poly(dUfl); poly(A) - poly(dUfl) + poly(U); and poly(A) + poly(U) + poly (dUfl). |
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Authors:
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E De Clercq; B Janik; R G Sommer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemico-biological interactions Volume: 14 ISSN: 0009-2797 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 1976 Jul |
Date Detail:
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Created Date: 1976-10-20 Completed Date: 1976-10-20 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 113-25 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Floxuridine / pharmacology Interferons / biosynthesis* Kidney / drug effects, metabolism Kinetics Nucleic Acid Hybridization Poly A / pharmacology Poly U / pharmacology Polynucleotides / pharmacology* Rabbits Ribonucleases / metabolism Temperature |
| Chemical | |
Reg. No./Substance:
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0/Polynucleotides; 24937-83-5/Poly A; 27416-86-0/Poly U; 50-91-9/Floxuridine; 9008-11-1/Interferons; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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