Document Detail


Biological and biochemical characteristics of murine megakaryoblastic cell line L8057.
MedLine Citation:
PMID:  7678813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biological and biochemical characteristics of a murine megakaryoblastic cell line (L8057), derived from an irradiated C3H/He mouse, were studied. The L8057 cells grew in liquid cultures with a doubling time of 24 to 27 hours. By light and electron microscopy, the L8057 cells were small and basophilic with a high nuclear/cytoplasmic ratio, but large cells (approximately 25 to 60 microns) were sometimes seen. Western blot analysis of cell extracts demonstrated that murine megakaryocyte and platelet antigens, glycoprotein (GP) IIb/IIIa, fibronectin, and factor VIII:RAg were expressed in L8057 cells. Acetylcholinesterase (AChE)-positive cells were rare (< 2.0%). Flow cytometric analysis demonstrated that 8.7% of the cells were spontaneously polyploid (> 4N DNA levels). In response to phorbol diester (TPA, 50 nm), L8057 cells showed a decrease in proliferation and a marked increase in cell size and amount of cytoplasm. The morphology of the cells became similar to that of megakaryocytes, with larger and lobulated nuclei and expansion of the demarcation membrane system compartment. The proportion of cells with ploidy levels greater than 4N increased to 40.8% (8N, 21.3 +/- 3.2%; 16N, 13.1 +/- 2.6%; > or = 32N, 6.4 +/- 4.9% [n = 4]). More than 90% of the cells became AChE-positive. TPA also increased the expression of GP IIb/IIIa, fibronectin and factor VIII:RAg. Thus, the L8057 cell line provides a potentially superior model with which to study the characteristics of megakaryocytic biology, differentiation and biochemistry.
Authors:
Y Ishida; J Levin; G Baker; P E Stenberg; Y Yamada; H Sasaki; T Inoue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Experimental hematology     Volume:  21     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-04     Completed Date:  1993-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  289-98     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, University of California School of Medicine, San Francisco.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / analysis,  genetics
Animals
Antigens, Surface / analysis,  genetics
Blotting, Western
Cell Differentiation / physiology
Cell Line
DNA / analysis,  genetics
Electrophoresis, Polyacrylamide Gel
Factor VIII / analysis,  genetics
Fibronectins / analysis,  genetics
Flow Cytometry
Hematopoietic Stem Cells / cytology*,  physiology,  ultrastructure
Megakaryocytes / cytology*,  physiology,  ultrastructure
Mice
Mice, Inbred C3H
Microscopy, Electron
Ploidies
Staining and Labeling
Tetradecanoylphorbol Acetate / pharmacology
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Fibronectins; 16561-29-8/Tetradecanoylphorbol Acetate; 9001-27-8/Factor VIII; 9007-49-2/DNA; EC 3.1.1.7/Acetylcholinesterase

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