Document Detail


Biological applications of microfluidic gradient devices.
MedLine Citation:
PMID:  20957276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Molecular gradients play an important role in diverse physiological and pathological phenomena such as immune response, wound healing, development and cancer metastasis. In the past 10 years, engineering tools have been increasingly used to develop experimental platforms that capture important aspects of cellular microenvironments to allow quantitative and reproducible characterization of cellular response to gradients. This review discusses the emergence of microfluidics-based gradient generators and their applications in enhancing our understanding of fundamental biological processes such as chemotaxis and morphogenesis. The principles and applications of microfluidic gradient generation in both 2D and 3D cellular microenvironments are discussed with emphasis on approaches to manipulate spatial and temporal distribution of signaling molecules.
Authors:
Sudong Kim; Hyung Joon Kim; Noo Li Jeon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-10-19
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  2     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-02     Completed Date:  2011-02-18     Revised Date:  2011-08-22    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  584-603     Citation Subset:  IM    
Affiliation:
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-744, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / physiology
Biomedical Engineering
Chemokines / physiology
Chemotaxis
Dictyostelium / physiology
Diffusion
Equipment Design
Escherichia coli / physiology
Extracellular Matrix / physiology
Humans
Hydrogels
Immunity
Microfluidic Analytical Techniques / instrumentation*,  methods
Models, Biological
Morphogenesis
Neoplasm Metastasis
Neovascularization, Physiologic
Signal Transduction
Stem Cells / cytology,  physiology
Chemical
Reg. No./Substance:
0/Chemokines; 0/Hydrogels

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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