Document Detail

Biological applications of atomic force microscopy.
MedLine Citation:
PMID:  8304408     Owner:  NLM     Status:  MEDLINE    
The newly developed atomic force microscope (AFM) provides a unique window to the microworld of cells, subcellular structures, and biomolecules. The AFM can image the three-dimensional structure of biological specimens in a physiological environment. This enables real-time biochemical and physiological processes to be monitored at a resolution similar to that obtained for the electron microscope. The process of image acquisition is such that the AFM can also measure forces at the molecular level. In addition, the AFM can interact with the sample, thereby manipulating the molecules in a defined manner--nanomanipulation! The AFM has been used to image living cells and the underlying cytoskeleton, chromatin and plasmids, ion channels, and a variety of membranes. Dynamic processes such as crystal growth and the polymerization of fibrinogen and physicochemical properties such as elasticity and viscosity in living cells have been studied. Nanomanipulations, including dissection of DNA, plasma membranes, and cells, and transfer of synthetic structures have been achieved. This review describes the operating principles, accomplishments, and the future promise of the AFM.
R Lal; S A John
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  The American journal of physiology     Volume:  266     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-10     Completed Date:  1994-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1-21     Citation Subset:  IM    
Department of Medicine, University of Chicago, Illinois 60637.
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MeSH Terms
Cell Membrane / ultrastructure
Cells / ultrastructure
Computer Systems
Macromolecular Substances
Membrane Proteins / ultrastructure
Microscopy / methods*
Reg. No./Substance:
0/Macromolecular Substances; 0/Membrane Proteins

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