| Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system. | |
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MedLine Citation:
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PMID: 20816730 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer with two intramolecular disulphide bonds essential for its biological activity. A mutated form of murine IL-10 was successfully expressed in E. coli, recovered and purified from inclusion bodies. Its ability to reduce tumor necrosis factor α synthesis and down-regulate class II major histocompatibility complex molecules expression on endotoxin-stimulated bone marrow-derived macrophages was confirmed, and shown to be similar to that of a commercially available IL-10. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems that can effectively deliver the protein. In this work it is shown that a dextrin nanogel effectively incorporate IL-10, stabilize, and enable the slow release of biologically active IL-10 over time. Altogether, these results demonstrate the suitability of dextrin nanogel to be used as a system for the controlled release of IL-10. |
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Authors:
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Vera Carvalho; Pedro Castanheira; Tiago Q Faria; Catarina Gonçalves; Pedro Madureira; Carlos Faro; Lucília Domingues; Rui M M Brito; Manuel Vilanova; Miguel Gama |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-09 |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 400 ISSN: 1873-3476 ISO Abbreviation: Int J Pharm Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-11 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 234-42 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Delayed-Action Preparations Dextrins / chemistry* Down-Regulation Drug Carriers / chemistry* Escherichia coli / genetics, metabolism Histocompatibility Antigens Class II / biosynthesis, genetics Interleukin-10 / administration & dosage*, genetics, isolation & purification Macrophages / drug effects, metabolism Mice Polyethylene Glycols / chemistry* Polyethyleneimine / chemistry* Recombinant Proteins / administration & dosage, genetics, isolation & purification Tumor Necrosis Factor-alpha / biosynthesis, genetics |
| Chemical | |
Reg. No./Substance:
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0/Delayed-Action Preparations; 0/Dextrins; 0/Drug Carriers; 0/Histocompatibility Antigens Class II; 0/NanoGel; 0/Polyethylene Glycols; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; 9002-98-6/Polyethyleneimine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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