Document Detail


Biological activity of a C-terminal fragment of Pasteurella multocida toxin.
MedLine Citation:
PMID:  11349023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The protein toxin of Pasteurella multocida PMT is a potent mitogen and activator of phospholipase Cbeta. In this study different toxin fragments were investigated. A C-terminal fragment encompassing amino acids 581 through 1285 (PMT581C) was constructed, which was inactive toward intact embryonic bovine lung (EBL) cells after addition to culture medium but caused reorganization of the actin cytoskeleton and rounding up of cells when introduced into the cells by electroporation. As the holotoxin, the toxin fragment PMT581C induced an increase in total inositol phosphate levels after introduction into the cell by electroporation. A C-terminal fragment shorter than PMT581C as well as N-terminal fragments were inactive. Exchange of cysteine-1165 for serine in the holotoxin resulted in a complete loss of the ability to increase inositol phosphate levels. Correspondingly, the mutated toxin fragment PMT581C.C1165S was inactive after cell introduction by electroporation, suggesting an essential role of Cys-1165 in the biological activity of the toxin.
Authors:
C Busch; J Orth; N Djouder; K Aktories
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  69     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-11     Completed Date:  2001-06-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3628-34     Citation Subset:  IM    
Affiliation:
Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Albert-Ludwigs-Universität Freiburg, D-79104 Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Bacterial Proteins*
Bacterial Toxins / chemistry*,  genetics,  toxicity*
Cattle
Cells, Cultured
Electroporation
Inositol Phosphates / metabolism
Lung / cytology,  embryology
Pasteurella multocida / metabolism
Recombinant Proteins
Chemical
Reg. No./Substance:
0/Actins; 0/Bacterial Proteins; 0/Bacterial Toxins; 0/Inositol Phosphates; 0/Pasteurella multocida toxin; 0/Recombinant Proteins
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